Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J73_RS03310 Genome accession   NZ_AP018780
Coordinates   661151..661690 (-) Length   179 a.a.
NCBI ID   WP_221253412.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-IV-1 isolate 13H36     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 656151..666690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J73_RS03280 (CHBNIV1_06180) fdx 656554..656895 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J73_RS03285 (CHBNIV1_06190) iscX 656895..657089 (+) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  K6J73_RS03290 (CHBNIV1_06200) - 657140..657754 (-) 615 WP_221253410.1 YfgM family protein -
  K6J73_RS03295 (CHBNIV1_06210) hisS 657772..659043 (-) 1272 WP_040034382.1 histidine--tRNA ligase -
  K6J73_RS03300 (CHBNIV1_06220) ispG 659053..660159 (-) 1107 WP_105897010.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J73_RS03305 (CHBNIV1_06230) - 660168..661079 (-) 912 WP_005656363.1 RodZ family helix-turn-helix domain-containing protein -
  K6J73_RS03310 (CHBNIV1_06240) pilF/pilF2 661151..661690 (-) 540 WP_221253412.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J73_RS03315 (CHBNIV1_06250) - 661791..662942 (-) 1152 WP_162627504.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J73_RS03320 (CHBNIV1_06260) - 663189..664067 (+) 879 WP_221253415.1 serine hydrolase -
  K6J73_RS03325 (CHBNIV1_06270) - 664433..665314 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  K6J73_RS03330 (CHBNIV1_06280) - 665314..666234 (+) 921 WP_040034370.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20584.16 Da        Isoelectric Point: 8.2626

>NTDB_id=71284 K6J73_RS03310 WP_221253412.1 661151..661690(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-IV-1 isolate 13H36]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSSQKIDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=71284 K6J73_RS03310 WP_221253412.1 661151..661690(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-IV-1 isolate 13H36]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCTCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCTCGCAAAAAATAGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.648

100

0.966
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment