Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J62_RS03345 Genome accession   NZ_AP018779
Coordinates   664762..665301 (-) Length   179 a.a.
NCBI ID   WP_038439288.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-8 isolate 10H15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 659762..670301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J62_RS03315 (CHBNIII8_06260) fdx 660165..660506 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J62_RS03320 (CHBNIII8_06270) iscX 660506..660700 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  K6J62_RS03325 (CHBNIII8_06280) - 660751..661365 (-) 615 WP_050848879.1 YfgM family protein -
  K6J62_RS03330 (CHBNIII8_06290) hisS 661383..662654 (-) 1272 WP_110431575.1 histidine--tRNA ligase -
  K6J62_RS03335 (CHBNIII8_06300) ispG 662664..663770 (-) 1107 WP_038439289.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J62_RS03340 (CHBNIII8_06310) - 663779..664690 (-) 912 WP_005666610.1 RodZ family helix-turn-helix domain-containing protein -
  K6J62_RS03345 (CHBNIII8_06320) pilF/pilF2 664762..665301 (-) 540 WP_038439288.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J62_RS03350 (CHBNIII8_06330) - 665402..666553 (-) 1152 WP_168770046.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J62_RS03355 (CHBNIII8_06340) - 666798..667676 (+) 879 WP_110431124.1 serine hydrolase -
  K6J62_RS03360 (CHBNIII8_06350) - 668042..668923 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  K6J62_RS03365 (CHBNIII8_06360) - 668923..669843 (+) 921 WP_005662663.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20583.32 Da        Isoelectric Point: 9.4145

>NTDB_id=71248 K6J62_RS03345 WP_038439288.1 664762..665301(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-8 isolate 10H15]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIKNAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQTDTFENIALCAYSAKKMNIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=71248 K6J62_RS03345 WP_038439288.1 664762..665301(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-8 isolate 10H15]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCAGCATCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAAAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAACAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGAATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

94.972

100

0.95
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment