Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NPA43_RS11155 Genome accession   NZ_CP101833
Coordinates   2200367..2201437 (-) Length   356 a.a.
NCBI ID   WP_099726649.1    Uniprot ID   A0A2G8IWD8
Organism   Bacillus pumilus strain NDY-10     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2195367..2206437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA43_RS11105 (NPA43_11105) sipW 2195468..2196040 (-) 573 WP_099726657.1 signal peptidase I SipW -
  NPA43_RS11110 (NPA43_11110) tapA 2196092..2196619 (-) 528 WP_230030384.1 amyloid fiber anchoring/assembly protein TapA -
  NPA43_RS11115 (NPA43_11115) - 2196871..2197197 (+) 327 WP_099726655.1 YqzG/YhdC family protein -
  NPA43_RS11120 (NPA43_11120) - 2197231..2197419 (-) 189 WP_008360237.1 YqzE family protein -
  NPA43_RS11125 (NPA43_11125) - 2197480..2197863 (-) 384 WP_099726654.1 competence type IV pilus minor pilin ComGG -
  NPA43_RS11130 (NPA43_11130) comGF 2197860..2198387 (-) 528 WP_256498811.1 competence type IV pilus minor pilin ComGF -
  NPA43_RS11135 (NPA43_11135) comGE 2198302..2198613 (-) 312 WP_099726652.1 competence type IV pilus minor pilin ComGE -
  NPA43_RS11140 (NPA43_11140) comGD 2198597..2199043 (-) 447 WP_230030386.1 competence type IV pilus minor pilin ComGD -
  NPA43_RS11145 (NPA43_11145) comGC 2199036..2199329 (-) 294 WP_008360227.1 competence type IV pilus major pilin ComGC Machinery gene
  NPA43_RS11150 (NPA43_11150) comGB 2199346..2200386 (-) 1041 WP_230030387.1 competence type IV pilus assembly protein ComGB -
  NPA43_RS11155 (NPA43_11155) comGA 2200367..2201437 (-) 1071 WP_099726649.1 competence type IV pilus ATPase ComGA Machinery gene
  NPA43_RS11160 (NPA43_11160) - 2201615..2202475 (-) 861 WP_256498812.1 STAS domain-containing protein -
  NPA43_RS11170 (NPA43_11170) - 2202701..2203081 (-) 381 WP_034319603.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NPA43_RS11175 (NPA43_11175) - 2203282..2203524 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  NPA43_RS11180 (NPA43_11180) - 2203573..2204211 (-) 639 WP_230030389.1 MBL fold metallo-hydrolase -
  NPA43_RS11185 (NPA43_11185) - 2204370..2204543 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  NPA43_RS11190 (NPA43_11190) - 2204568..2204882 (-) 315 WP_099726646.1 MTH1187 family thiamine-binding protein -
  NPA43_RS11195 (NPA43_11195) - 2204960..2206213 (-) 1254 WP_230030391.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40262.62 Da        Isoelectric Point: 9.0358

>NTDB_id=712237 NPA43_RS11155 WP_099726649.1 2200367..2201437(-) (comGA) [Bacillus pumilus strain NDY-10]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRAIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRTGQVFVRMSTLPTVNDESLVIRILPQDHIPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETAKTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=712237 NPA43_RS11155 WP_099726649.1 2200367..2201437(-) (comGA) [Bacillus pumilus strain NDY-10]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTTCATATTGTCCC
AAAAGAAAAGGAAGCTTCGGTATCTTTCCGTGTAGACTCAGATTTAATCCAGCAGCGAGCCATTGATAAAAAAAGCGGGG
AACGGTTAATTGCTCATTTTAAATTTTTATCCTCCATGGATATCGGCGAAAAAAGGAGACCGCAAAACGGGTCATTGGCT
GTGATGCTGAGAACTGGCCAAGTGTTTGTTCGAATGTCTACATTACCGACTGTAAATGATGAAAGTTTAGTGATTAGGAT
TTTACCGCAGGATCATATCCCGAAAACAAAATATCTGTCGTTATTTCCGAAAGCCTCAGCCAGATTATTATCATTTTTGA
ATCATTCGCATGGACTCATTTTATTTACTGGGCCAACCAATTCAGGGAAAACAACAACACTTTATTCATTGATACAGTTT
GCAAAAAAGCATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTACAGGTACA
AGTCAATGAAAAAGCAGGCATCACGTATGCGGCGGGATTACGCGCTATTTTAAGACATGACCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACAGCCAAAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACGCTGCAT
GCGAAAAATGCAAAAGGTGCCCTTTACCGAATGCTTGAATTTGGTGTGACGATGAATGAGCTCGAACAAACAATGGTTGC
GATTGCTGCACAGCGATTAATCGAGCTCACGTGTCCATTTTGCGGGGAAACGTGTCAGCTATATTGCAAATTAAATCGAC
CGGTCAGACGAACGAATGTTTTTGAATTACTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCCAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTGGGCTATCTATCGAAAAACACATATCA
TCGCTGGGTTTATGAAGAAGCGAGTCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G8IWD8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649