Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   NPA43_RS05180 Genome accession   NZ_CP101833
Coordinates   1053424..1057128 (+) Length   1234 a.a.
NCBI ID   WP_256499448.1    Uniprot ID   -
Organism   Bacillus pumilus strain NDY-10     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1048424..1062128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA43_RS05165 (NPA43_05165) - 1048719..1049129 (+) 411 WP_249705426.1 DoxX family protein -
  NPA43_RS05170 (NPA43_05170) - 1049324..1049770 (-) 447 WP_249705427.1 ferritin-like domain-containing protein -
  NPA43_RS05175 (NPA43_05175) addB 1049937..1053446 (+) 3510 WP_256499447.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NPA43_RS05180 (NPA43_05180) addA 1053424..1057128 (+) 3705 WP_256499448.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NPA43_RS05185 (NPA43_05185) sbcD 1057155..1058330 (+) 1176 WP_099728140.1 exonuclease subunit SbcD -
  NPA43_RS05190 (NPA43_05190) sbcC 1058327..1061722 (+) 3396 WP_230030940.1 exonuclease subunit SbcC -
  NPA43_RS05195 (NPA43_05195) - 1061742..1062041 (+) 300 WP_230030939.1 HNH endonuclease -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142565.66 Da        Isoelectric Point: 5.0982

>NTDB_id=712215 NPA43_RS05180 WP_256499448.1 1053424..1057128(+) (addA) [Bacillus pumilus strain NDY-10]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEKLPFYPYIREDLSLVFRSCQELLERA
LTLSKEPGGPAPRAENFIDDLEQVHELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATEARNQAKKQLEKLK
DEYFMRSPAQHLNSLAEMKPVVETLVELVIQFGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHPAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKHFKTDGNETGKRIDLNKNFR
SRSDVLDCTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSKDTTTEMLLVHLDQHEAESDEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALMRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRFLRKWRDFSMNHSVAELIWEVY
RDTQYVDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGEEWLLPDFERYQAKTYLDFIGPALIRHQAMSSILEEAGDVVLSHPSSFTISFTQASDL
LKEDVSLEKKQHDEVVQALMEGLPVEGRGDYEEEVAERLSWKYPYVAASQVGTKQSVSEIKRMKEVQDEYSVPASIRKAR
ATLYDRPAFMKKKTLTAAEQGTAMHTVMQHIPLPSEEPYDESRIEQLLDSLQQRDLLTDEQILSIDREGIMAFFSTSIGQ
KLRKADWVKREVSFSMILPVKEVYSHIDAEDEPVLIQGMIDCLFEVDGKLYLLDYKTDRVQGRYSGGLEAAESILKKRYE
TQLALYAAAVERLTNRSLEGKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=712215 NPA43_RS05180 WP_256499448.1 1053424..1057128(+) (addA) [Bacillus pumilus strain NDY-10]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAATGGGAAGCCATCGTTTCAGAAGGACAGGATATCCT
TGTTGCTGCTGCGGCGGGGTCAGGTAAAACGGCTGTCTTGGTTGAACGGCTGATTCGAAAAATGACCCGGCCTGAGGATC
CAGTTGACGTAGACCGTCTGCTCGTTGTGACTTTTACAAATGCATCTGCGGCAGAAATGAAGCACCGGATCACTGAAGCA
CTTGAAAAAGAGTTATCCAAAAACCCAGGGTCACTTCATATGAGAAGACAGCTGTCCCTCATGAATCGAGCCAATATTTC
TACCTTGCACTCCTTTTGCTTGCAAGTTCTACGCACCTTTTATTATGAGATTGATCTTGATCCAGGCTTCCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAGCTGTTTGAAGATGAATATAAGGCTGGAAAGCCAGCA
TTCTTTGAACTGGTTGATCGTTACACATCGGATCGGCATGACTTAGATTTGCAATGGCTCGTGAAAAGAATTTATGAATT
TTCTAGGTCTCATCCTTCTCCGGAGCAATGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATGCACAAACAAAAGTAG
AAAAATTACCATTTTATCCATATATAAGAGAGGATCTTTCTCTCGTTTTCCGGAGCTGTCAGGAGCTGCTCGAACGAGCT
CTTACCCTATCAAAAGAGCCGGGTGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTACATGAATT
AATCAGGCATCAAGATGATTTTGAGAAGCTTTATGAGCTCTTACCGAACATCAATTTTAAAAGGCTAAAGACGTGCAAAG
GGGACGAATACGACCCTGTTCTGTTAGAAAAAGCCACAGAGGCACGCAATCAAGCGAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCTGCACAGCATTTAAATAGCTTGGCTGAAATGAAGCCAGTTGTTGAGACCCTTGTGGA
GCTTGTCATCCAATTTGGAGAACGTTTCGAAAGAGCAAAACAAGAAAAATCAATCGTAGACTTTTCAGATTTAGAGCATT
ATTGCTTACGCATTTTAGCGAAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCAGCTAAATACTATCAACAGCAA
TTTGAAGAAGTGCTCGTTGATGAATACCAGGATACAAACCTTGTACAAGAAACGATTTTAAAGCTTGTGTCTAAAGGGGA
ACATCCTGCAGAAGGCAACCTTTTTATGGTTGGTGATGTAAAGCAGTCCATTTATCGGTTTAGACTGGCTGAGCCAATGC
TCTTTTTAAACAAATATAAACACTTTAAAACAGACGGCAATGAAACAGGTAAGAGAATTGATTTAAATAAAAACTTCCGT
AGCCGTTCTGATGTTTTAGACTGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGGGCGAGTTATCCGCAAAGCAAAGATACAACGACAGAAATGCTCCTTGTCCATCTAG
ATCAACATGAAGCGGAAAGCGACGAAGAACGAGAAGAACTAGAGACCGTGCAATTTGAGGCGAGGGTCATTGCAAAGAAA
ATAAAAGAATTGGTTGAACAGCCTTTTCAAGTGTACGATGCAAAACAGCAAATGACCCGCAACTTGCAATATCGAGATAT
CGTGATTTTGCTTCGTTCTATGCCGTGGGCCCCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCGTCAGGCTATTTCGAAGCAACAGAGGTGTCTGTGATTCTTTCTCTGTTAAAAGTAATCGATAATCCATATCAG
GATATCCCGCTTGCAGCTGTTTTAAGATCACCTATTGTTCATTTAGACGAAAATGAAATGGCACTTATGAGAACGAGTGA
TAAAAAAGGCACTTATTATGATGCTGTGAAGGCATTCATGAGTGTAACCCATTCTGATCACCCTACATGCAAAAAACTAG
AGAGATTCTTCCGATTTCTGCGTAAGTGGCGTGATTTCTCCATGAATCATTCGGTAGCAGAATTGATTTGGGAAGTGTAC
CGCGATACCCAGTATGTAGATTATGTTGGCGGGATGCCTGGCGGGAAGCAGAGGCAGGCAAATCTTCGTGCTCTTTATGA
TCGAGCAAAGCAGTATGAAAAGGCTGCCTTTAGAGGACTGTTTCGATTCCTTCGTTTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCAGCGAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATTCACAGCAGTAAAGGT
TTAGAATTTCCAGTTGTCTTTACCGCAGGACTCGGCAGGAACTTTAATATGATGGACTTAAACCAGTCCTACTTGCTCGA
TAAAGAACTAGGCTTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCGACATTACCGCTTGTGGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAGCTGAGGGTTCTATACGTGGCGCTTACAAGGGCCAAGGAAAAATTA
TTCCTTGTCGGCTCCGTCAAAAATCAGGTGAAAGCATTAAGTAAATGGCAAAATGCCGCAAATGGAGAAGAGTGGCTCCT
CCCTGATTTTGAACGATACCAAGCCAAAACTTACTTAGATTTTATAGGACCTGCTCTCATCCGTCATCAAGCGATGTCAT
CCATTTTAGAAGAAGCAGGCGATGTTGTTCTCTCACATCCATCATCATTCACCATCTCATTTACTCAAGCCTCAGATCTT
CTAAAAGAAGATGTGTCACTTGAAAAGAAGCAGCATGACGAAGTGGTACAAGCATTAATGGAAGGTCTTCCTGTTGAAGG
ACGTGGTGATTATGAAGAGGAGGTTGCCGAGAGACTCTCATGGAAATACCCATATGTAGCGGCCTCGCAAGTCGGTACAA
AGCAGTCTGTCTCAGAGATAAAAAGGATGAAAGAAGTACAGGATGAGTACAGTGTTCCTGCTTCTATTCGAAAAGCTCGT
GCAACCTTGTATGACCGGCCGGCTTTTATGAAGAAAAAAACACTCACTGCCGCAGAACAAGGTACTGCGATGCATACAGT
CATGCAGCACATTCCGCTTCCTTCAGAAGAGCCTTATGATGAATCTCGTATAGAGCAGCTGCTCGATTCCTTGCAGCAGC
GAGACTTACTAACAGATGAGCAAATCCTGTCTATTGATCGAGAAGGGATCATGGCCTTCTTTTCTACTTCTATTGGTCAA
AAACTGCGGAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGATTTTACCTGTCAAAGAGGTGTACAGCCATAT
TGATGCAGAGGATGAACCTGTGCTTATTCAAGGAATGATTGATTGTTTGTTTGAAGTAGATGGAAAGCTTTATTTACTTG
ATTATAAAACAGATAGAGTGCAAGGCAGATATTCTGGCGGACTAGAGGCTGCCGAATCGATTTTGAAAAAGCGCTATGAA
ACCCAATTAGCGTTATACGCAGCAGCAGTAGAACGTTTAACAAACAGATCATTAGAGGGGAAAATTCTCTATTTCTTCGA
TGGGAATTTAGAAATTTCTTTATAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

65.966

100

0.661