Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   NPA43_RS05175 Genome accession   NZ_CP101833
Coordinates   1049937..1053446 (+) Length   1169 a.a.
NCBI ID   WP_256499447.1    Uniprot ID   -
Organism   Bacillus pumilus strain NDY-10     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1044937..1058446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA43_RS05145 (NPA43_05145) lepB 1045425..1045937 (+) 513 WP_230030944.1 signal peptidase I -
  NPA43_RS05150 (NPA43_05150) - 1045974..1047119 (-) 1146 WP_099728134.1 AbrB family transcriptional regulator -
  NPA43_RS05155 (NPA43_05155) - 1047408..1047830 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  NPA43_RS05160 (NPA43_05160) - 1047823..1048662 (+) 840 WP_230030943.1 M56 family metallopeptidase -
  NPA43_RS05165 (NPA43_05165) - 1048719..1049129 (+) 411 WP_249705426.1 DoxX family protein -
  NPA43_RS05170 (NPA43_05170) - 1049324..1049770 (-) 447 WP_249705427.1 ferritin-like domain-containing protein -
  NPA43_RS05175 (NPA43_05175) addB 1049937..1053446 (+) 3510 WP_256499447.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NPA43_RS05180 (NPA43_05180) addA 1053424..1057128 (+) 3705 WP_256499448.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NPA43_RS05185 (NPA43_05185) sbcD 1057155..1058330 (+) 1176 WP_099728140.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 135044.04 Da        Isoelectric Point: 5.2029

>NTDB_id=712214 NPA43_RS05175 WP_256499447.1 1049937..1053446(+) (addB) [Bacillus pumilus strain NDY-10]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSDELKGAHIYIDGFYQFTPQQLLVIEQLLLHATKVTAAFTVDRS
YHDTQPNELDLFRMTGKTYFQLYQLAKECGADISENILERNKRHLHTPDLAYLENQYEQRPPQPYQEETPHLTVSKSANK
RAEIEGVARDILDLVREDGFRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNSSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLFQLQKRLKKAQKVRDMVEAIYVFLEEIQVPDKLEKARLEAEETGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSRHLYLSYSASDEEGRSLLPSTFIKYCQELMPNHQHALYVLDPEQLDDEDQLKFIANEH
VSLSYTVSQLQQWLNQYPISDVWWSVYNDLMTSPNRDVSKKIMSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLIEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGMEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGLSQVIQAGLKKDGSFRSDSAVLSEDHFHILTKHVRRTFKEAGERITNGEVEINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLTSEKDDVMIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=712214 NPA43_RS05175 WP_256499447.1 1049937..1053446(+) (addB) [Bacillus pumilus strain NDY-10]
GTGGAAATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAGATAAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGAATGATTAGGGCCAAAGTATTTAGTTTTACTCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTCACAAGCACAGGGATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGAAAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTTGAGCAAATTGAAAAAACGATGGCTGAATTCAAAAGGTACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCAGCGTCCAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCAGAA
AAACTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGATTATTT
GACACTTCTTACGCAGCAAATTCCTTTATCTGATGAACTAAAAGGAGCGCATATTTATATAGATGGTTTTTATCAGTTTA
CCCCGCAGCAGCTTCTCGTCATTGAGCAGCTCCTGCTGCATGCCACAAAAGTAACCGCTGCTTTCACAGTGGATCGTTCA
TATCATGACACGCAGCCAAATGAACTTGATCTATTTCGCATGACAGGCAAAACGTATTTTCAACTCTATCAGCTTGCCAA
AGAGTGCGGAGCGGACATTTCTGAGAACATTTTGGAGAGAAATAAACGGCATCTTCATACACCAGACCTAGCTTATTTAG
AAAATCAATATGAGCAGAGACCGCCGCAGCCGTATCAAGAAGAAACGCCTCATCTCACAGTATCTAAAAGTGCAAACAAA
CGAGCTGAGATTGAGGGAGTAGCGAGAGACATTCTTGATTTAGTGAGAGAGGACGGCTTCAGGTTACGAGATATTTCGAT
TGTTGCAAGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGACGTCATAAAAGGGAACTGGCGGTAT
GAAGCAGTGTTTCGCTGTGTGAAAACAGAGTTCCTATTTCCGCTAGAACTCGCTAAGAACAAAGCGAGAGAACAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTATGGTGTAAAAGGGGAACGCTGGACAAACAGCTCCCGTTTTCATTACAGGCGAT
TCCAATCATTAGATGAAGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTGAATGACGTGAAAGAATGG
ATTGTTCCCCCTCTTTTTCAATTGCAAAAAAGATTGAAAAAAGCACAAAAGGTGAGGGACATGGTAGAGGCCATCTATGT
CTTTCTAGAGGAAATACAAGTGCCGGATAAGCTTGAAAAGGCAAGGCTTGAGGCTGAAGAAACAGGTAGATTAGCTGAAG
CGATGCAGCATGGACAAGTATGGGATGCTGTTATTCAATTAATGGATGAATTTGTTGAAATGCTCGGAGAAGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGTCTAGCATCTCTAAAGTTTGCTTTGATTCCGCCTTCACTTGACCA
AGTATTTATCGGAAGTATGGATCTATCTAGAATGTATCAAGTGAAGTGCACGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCAGCGCGTCCTTCTGATGAAAGTGTATTGTCTGAGGATGACCGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTGGCA
GAGACAGGGAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCGTCATTTGTATCT
ATCCTATTCGGCTTCAGATGAAGAAGGACGTTCTCTATTGCCTTCGACTTTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCACCAGCATGCTCTTTATGTGTTAGATCCAGAACAGCTGGATGACGAGGATCAATTAAAATTTATAGCGAATGAGCAT
GTCTCGTTATCCTATACCGTCTCGCAGCTGCAGCAATGGCTCAATCAATATCCCATCAGCGATGTATGGTGGAGTGTATA
TAATGATTTAATGACGTCACCCAATCGGGATGTATCAAAAAAGATCATGTCAAGTTTGTTTTTTACAAATAGAGCGAAGC
GCTTAAAGCCAAATGTCACAAAAGAGCTTTACGGTGATCATATTCAAGGCAGTGTTTCAAGGATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCTCACGGGTTAAAACTAAAGGATCGACAATTTTACAAATTGGAAGCACCCGATATCGG
TCAGCTGTTTCACTCAGCTTTAAAACATATTTCAGATACGCTCATTGAACAAAAAAAGGATTGGAAGAACTTAACGAAGG
AAGATTGTGTCACCTACTCAAGACATGCGATTGAACAGCTTGCTCCGCGTCTGCAAAAGGAAATTTTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAACTTCAGAAGATCCTGATTCGCGTCTCTTCTATATTAAGTGAACATGCCAAAGTGAG
TGGATTTTCTCCAGTTGGACTTGAATTAGGTTTTGGAGGTCAAGGGCCTCTGCCGCCGTTTACTTTTCAATTGAAAAATG
GCTGTACGATGGAGCTTGTCGGAAGAATAGACCGAGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTTTTGAGAATTGTC
GACTATAAGTCCAGCGAGAAAGGGCTTGATTTAGCAGAGGTATACTATGGCTTGGCCTTGCAAATGCTCACTTACTTAGA
CCTGACAATTACTTATTCTAAAGAATGGCTAGGCATGGAGGCGACTCCTGCTGGAATTTTATATTTCCATATTCATGATC
CTCTCATTCAAGCGCCTATTCCGCTCGCAGAGGATGAAATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGTCTG
CTGCTTGAAGATGTAGAAGCAGTAAAACTAATGGATCAAACGCTTGAGTCAGGATTATCCCAAGTCATTCAAGCGGGCTT
GAAAAAGGATGGATCTTTCCGTTCGGATTCTGCAGTTTTAAGTGAAGATCATTTCCACATACTCACCAAACACGTTCGGC
GCACTTTTAAAGAGGCAGGAGAAAGAATTACGAACGGTGAGGTAGAAATTAATCCATATAAATTGAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAGTTTGATGAATCAATAGAGGACAATGAATTTAGAGTGCTCACATCAGA
GAAAGATGATGTCATGATAGACCGGATCAAAAAAGAAGGAGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.15

99.316

0.657