Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NPA43_RS00590 Genome accession   NZ_CP101833
Coordinates   109707..111086 (+) Length   459 a.a.
NCBI ID   WP_105928548.1    Uniprot ID   -
Organism   Bacillus pumilus strain NDY-10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104707..116086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA43_RS00570 (NPA43_00570) - 105049..105513 (+) 465 WP_034323278.1 CtsR family transcriptional regulator -
  NPA43_RS00575 (NPA43_00575) - 105528..106085 (+) 558 WP_180275544.1 UvrB/UvrC motif-containing protein -
  NPA43_RS00580 (NPA43_00580) - 106090..107181 (+) 1092 WP_099728204.1 protein arginine kinase -
  NPA43_RS00585 (NPA43_00585) clpC 107178..109613 (+) 2436 WP_034323269.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  NPA43_RS00590 (NPA43_00590) radA 109707..111086 (+) 1380 WP_105928548.1 DNA repair protein RadA Machinery gene
  NPA43_RS00595 (NPA43_00595) disA 111089..112168 (+) 1080 WP_099728202.1 DNA integrity scanning diadenylate cyclase DisA -
  NPA43_RS00600 (NPA43_00600) - 112322..113422 (+) 1101 WP_256499213.1 PIN/TRAM domain-containing protein -
  NPA43_RS00605 (NPA43_00605) ispD 113436..114125 (+) 690 WP_099728201.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NPA43_RS00610 (NPA43_00610) ispF 114129..114605 (+) 477 WP_099728200.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50001.42 Da        Isoelectric Point: 7.0709

>NTDB_id=712204 NPA43_RS00590 WP_105928548.1 109707..111086(+) (radA) [Bacillus pumilus strain NDY-10]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDQNQNVLYISGEESIKQTKLRADRLGIDSTSLHVLAETDMEYIT
SSIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPQPADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=712204 NPA43_RS00590 WP_105928548.1 109707..111086(+) (radA) [Bacillus pumilus strain NDY-10]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCTGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAAACCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATTTCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTCGTTCTCATTGGCGGAGACCCTGGGATTGGGAAGTCCACATT
ATTACTACAAGTATCAGCACAGCTCTCAGACCAAAATCAGAATGTGTTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGATCGCCTCGGCATTGATAGCACCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTTCTATACAAGAGATGAAACCCGCTTTTGTTGTGGTGGATTCGATTCAAACCGTTTATCAAAGCGATATAACGTCAGC
TCCTGGATCTGTTTCTCAGGTAAGAGAATGCACAGCGCAGCTGATGAAGATTGCCAAGACGAATGGTATTCCGATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGTCCACGTCTTTTAGAGCATATGGTGGACACGGTTCTTTATTTT
GAAGGGGAGCGGCATCATACGTTTCGTATCTTACGAGCAGTGAAAAACCGATTTGGTTCAACGAATGAATTAGGGATTTT
TGAAATGAGAGAAGAAGGACTCACGGAAGTATTAAACCCATCAGAAATTTTTTTAGAAGAGCGGTCAGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCCGTTCTTGTGGAGATACAAGCATTAATTTCGCCGACAAGCTTT
GGGAATCCACGGAGAATGGCTACAGGTCTTGATCATAATCGTGTGTCATTGCTAATGGCCGTTTTAGAAAAACGTGTAGG
GCTGCTGCTGCAAAACCAAGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCAGCGATTGACTTGGCCA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGACGCAGCGCCGCAACCAGCGGATTGCTTTATAGGAGAAGTTGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCTAAACTAGGATTTAAGCGAATGTTTATTCC
TCAGGCGAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGCTAGTCGGTGTAGAAAATGTAGCGGAGGCACTTCGTA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.336

99.782

0.871
  radA Streptococcus mitis NCTC 12261

62.031

98.693

0.612
  radA Streptococcus pneumoniae Rx1

62.031

98.693

0.612
  radA Streptococcus pneumoniae D39

62.031

98.693

0.612
  radA Streptococcus pneumoniae R6

62.031

98.693

0.612
  radA Streptococcus pneumoniae TIGR4

62.031

98.693

0.612
  radA Streptococcus mitis SK321

62.031

98.693

0.612