Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J60_RS03530 Genome accession   NZ_AP018778
Coordinates   688379..688918 (-) Length   179 a.a.
NCBI ID   WP_005654843.1    Uniprot ID   A0A0H3PFD4
Organism   Haemophilus influenzae strain CHBN-III-7 isolate 15H01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 683379..693918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J60_RS03500 (CHBNIII7_06610) fdx 683728..684069 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J60_RS03505 (CHBNIII7_06620) iscX 684069..684263 (+) 195 WP_005630102.1 Fe-S cluster assembly protein IscX -
  K6J60_RS03510 (CHBNIII7_06630) - 684314..684928 (-) 615 WP_005669110.1 YfgM family protein -
  K6J60_RS03515 (CHBNIII7_06640) hisS 684946..686217 (-) 1272 WP_005669108.1 histidine--tRNA ligase -
  K6J60_RS03520 (CHBNIII7_06650) ispG 686227..687333 (-) 1107 WP_014550444.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J60_RS03525 (CHBNIII7_06660) - 687342..688307 (-) 966 WP_221267452.1 RodZ family helix-turn-helix domain-containing protein -
  K6J60_RS03530 (CHBNIII7_06670) pilF/pilF2 688379..688918 (-) 540 WP_005654843.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J60_RS03535 (CHBNIII7_06680) - 689019..690170 (-) 1152 WP_005662659.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J60_RS03540 (CHBNIII7_06690) - 690417..691295 (+) 879 WP_118807481.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K6J60_RS03545 (CHBNIII7_06700) - 691277..691414 (-) 138 WP_192885197.1 hypothetical protein -
  K6J60_RS03550 (CHBNIII7_06710) - 691661..692542 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  K6J60_RS03555 (CHBNIII7_06720) - 692542..693462 (+) 921 WP_118807480.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20586.20 Da        Isoelectric Point: 8.2626

>NTDB_id=71213 K6J60_RS03530 WP_005654843.1 688379..688918(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-7 isolate 15H01]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=71213 K6J60_RS03530 WP_005654843.1 688379..688918(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-7 isolate 15H01]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PFD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment