Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J69_RS04170 Genome accession   NZ_AP018777
Coordinates   830825..831364 (-) Length   179 a.a.
NCBI ID   WP_110431126.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-6 isolate 13H31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 825825..836364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J69_RS04140 (CHBNIII6_07890) fdx 826230..826571 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J69_RS04145 (CHBNIII6_07900) iscX 826571..826765 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  K6J69_RS04150 (CHBNIII6_07910) - 826814..827428 (-) 615 WP_110431129.1 YfgM family protein -
  K6J69_RS04155 (CHBNIII6_07920) hisS 827446..828717 (-) 1272 WP_110431128.1 histidine--tRNA ligase -
  K6J69_RS04160 (CHBNIII6_07930) ispG 828727..829833 (-) 1107 WP_050822779.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J69_RS04165 (CHBNIII6_07940) - 829842..830753 (-) 912 WP_110431127.1 RodZ family helix-turn-helix domain-containing protein -
  K6J69_RS04170 (CHBNIII6_07950) pilF/pilF2 830825..831364 (-) 540 WP_110431126.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J69_RS04175 (CHBNIII6_07960) - 831465..832616 (-) 1152 WP_168770046.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J69_RS04180 (CHBNIII6_07970) - 832861..833739 (+) 879 WP_110431124.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K6J69_RS04185 (CHBNIII6_07980) - 833886..835022 (-) 1137 WP_221257498.1 RNA-guided endonuclease TnpB family protein -
  K6J69_RS04190 (CHBNIII6_07990) - 835397..836278 (+) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20583.28 Da        Isoelectric Point: 8.6194

>NTDB_id=71185 K6J69_RS04170 WP_110431126.1 830825..831364(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-6 isolate 13H31]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=71185 K6J69_RS04170 WP_110431126.1 830825..831364(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-6 isolate 13H31]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCAGCATCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(73-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.207

100

0.972
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment