Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NPA28_RS12750 Genome accession   NZ_CP101718
Coordinates   2524640..2525710 (-) Length   356 a.a.
NCBI ID   WP_217827782.1    Uniprot ID   -
Organism   Bacillus halotolerans strain S-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2519640..2530710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA28_RS12705 (NPA28_12705) tapA 2520115..2520876 (-) 762 WP_217827776.1 amyloid fiber anchoring/assembly protein TapA -
  NPA28_RS12710 (NPA28_12710) - 2521153..2521476 (+) 324 WP_024122040.1 YqzG/YhdC family protein -
  NPA28_RS12715 (NPA28_12715) - 2521519..2521698 (-) 180 WP_003236949.1 YqzE family protein -
  NPA28_RS12720 (NPA28_12720) comGG 2521770..2522144 (-) 375 WP_217827777.1 competence type IV pilus minor pilin ComGG Machinery gene
  NPA28_RS12725 (NPA28_12725) comGF 2522145..2522528 (-) 384 WP_217827778.1 competence type IV pilus minor pilin ComGF Machinery gene
  NPA28_RS12730 (NPA28_12730) comGE 2522554..2522901 (-) 348 WP_217827779.1 competence type IV pilus minor pilin ComGE Machinery gene
  NPA28_RS12735 (NPA28_12735) comGD 2522885..2523316 (-) 432 WP_217827780.1 competence type IV pilus minor pilin ComGD Machinery gene
  NPA28_RS12740 (NPA28_12740) comGC 2523306..2523602 (-) 297 WP_010334925.1 competence type IV pilus major pilin ComGC Machinery gene
  NPA28_RS12745 (NPA28_12745) comGB 2523616..2524653 (-) 1038 WP_217827781.1 competence type IV pilus assembly protein ComGB Machinery gene
  NPA28_RS12750 (NPA28_12750) comGA 2524640..2525710 (-) 1071 WP_217827782.1 competence protein ComGA Machinery gene
  NPA28_RS12755 (NPA28_12755) - 2526032..2526442 (-) 411 WP_217827783.1 CBS domain-containing protein -
  NPA28_RS12760 (NPA28_12760) corA 2526505..2527458 (-) 954 WP_217827784.1 magnesium transporter CorA -
  NPA28_RS12765 (NPA28_12765) - 2527602..2528931 (+) 1330 Protein_2478 hemolysin family protein -
  NPA28_RS12770 (NPA28_12770) - 2529011..2529850 (-) 840 WP_044154573.1 STAS domain-containing protein -
  NPA28_RS12780 (NPA28_12780) mgsR 2530083..2530463 (-) 381 WP_052330300.1 transcriptional regulator MgsR -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40498.66 Da        Isoelectric Point: 8.8394

>NTDB_id=711768 NPA28_RS12750 WP_217827782.1 2524640..2525710(-) (comGA) [Bacillus halotolerans strain S-5]
MDSIEKTSKTLIEEAYRTKASDIHIVPRERDAIIHFRVDHALLKKRNMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGDVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLIQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAQIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLSCPFCEDGCTSVYCRLSRNVRRASVYELLYGKNLQQCIQEAKGD
NANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHETD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=711768 NPA28_RS12750 WP_217827782.1 2524640..2525710(-) (comGA) [Bacillus halotolerans strain S-5]
GTGGATTCGATAGAAAAAACAAGCAAAACTTTAATTGAAGAGGCATATAGGACGAAGGCCTCTGACATTCACATTGTGCC
GAGGGAACGGGACGCAATCATTCATTTTCGGGTTGACCATGCATTGCTGAAAAAGCGAAACATGAAAAAAGAAGAGTGTG
TAAGGCTCATTTCTCATTTTAAGTTTCTCTCAGCTATGGACATAGGGGAAAGAAGAAAGCCGCAAAATGGATCTCTTACT
CTAAAATTAAAAGAGGGTGACGTTCACTTAAGGATGTCAACGCTCCCGACAATTAACGAAGAGAGCCTCGTCATCAGAGT
GATGCCTCAGTATAATATCCCTTCGATTGACAAACTGTCTTTATTCCCAAAGACAGGAGCCACCTTGCTTTCATTTTTAA
AACACTCCCACGGCATGCTGATTTTTACCGGACCAACAGGCTCTGGGAAAACAACAACATTATATTCTCTGATCCAATAT
GCAAAAAAACATTTTAATCGAAACATTGTCACACTTGAAGATCCAGTTGAAACGAGGGATGAAGATGTTCTTCAAGTGCA
GGTGAATGAAAAAGCCGGTGTCACATATTCCACAGGGCTGAAGGCAATTTTGCGCCATGACCCCGATATGATCATTTTAG
GTGAGATTAGAGACGCTGAAACAGCACAAATCGCGGTGCGGGCAGCGATGACTGGTCATTTAGTGCTGACGAGCCTTCAT
ACGAGAGATGCGAAGGGCGCGATTTACAGGCTGCTTGAATTTGGTATTAATATGAATGAAATCGAGCAAACGGTTATTGC
GATAGCGGCTCAGCGTTTAGTTGATTTGTCTTGCCCGTTTTGTGAAGACGGATGTACATCAGTGTATTGCCGCCTGTCAC
GAAATGTCAGGAGAGCAAGCGTATACGAGCTGCTTTACGGGAAAAACCTTCAGCAGTGTATCCAGGAAGCGAAAGGGGAC
AATGCAAATTACCAATATCAAACGCTGCGTCAAATTATAAGAAAAGGGATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAACAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

95.506

100

0.955