Detailed information NTG0711409

Bioinformatically predicted

Overview

• Name: recF
• Type: Machinery gene
• Locus tag: POF61_RS10140
• Genome accession: NZ_CP116709
• Coordinates: 1991000..1992109 (+)
• Length: 369 a.a.
• NCBI ID: WP_000264872.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1986000..1997109

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF61_RS10115 (POF61_10115)	-	1986372..1987211 (-)	840	WP_000181757.1	energy-coupling factor ABC transporter ATP-binding protein	-
POF61_RS10120 (POF61_10120)	pgsA	1987211..1987753 (-)	543	WP_000239225.1	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	-
POF61_RS10125 (POF61_10125)	-	1987876..1989159 (-)	1284	WP_000217764.1	pitrilysin family protein	-
POF61_RS10130 (POF61_10130)	-	1989161..1990405 (-)	1245	WP_000706188.1	pitrilysin family protein	-
POF61_RS10135 (POF61_10135)	yaaA	1990644..1990997 (+)	354	WP_000384867.1	S4 domain-containing protein YaaA	-
POF61_RS10140 (POF61_10140)	recF	1991000..1992109 (+)	1110	WP_000264872.1	DNA replication/repair protein RecF	Machinery gene
POF61_RS10145 (POF61_10145)	-	1992121..1992987 (-)	867	WP_000398683.1	GRP family sugar transporter	-
POF61_RS10150 (POF61_10150)	-	1993043..1993705 (-)	663	WP_001865840.1	helix-turn-helix transcriptional regulator	-
POF61_RS10155 (POF61_10155)	guaB	1993780..1995261 (-)	1482	WP_000073440.1	IMP dehydrogenase	-
POF61_RS10160 (POF61_10160)	-	1995418..1995903 (-)	486	WP_001034427.1	arginine repressor	-
POF61_RS10165 (POF61_10165)	-	1995913..1996593 (-)	681	WP_000628848.1	Crp/Fnr family transcriptional regulator	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 42470.29 Da
• Isoelectric Point: 7.5270

>NTDB_id=711409 POF61_RS10140 WP_000264872.1 1991000..1992109(+) (recF) [Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant]
MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIYFLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGH
LSLNIQLSEKGRITKVNHLKQAKLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKPTYLAELSNYNHVLKQR
NTYLKTTNNVDKTFLSVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHLSIHYKSSIEFTDKSSIREHFLN
QLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFGSQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQ
LKLLEGIKENVQTFITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD

Nucleotide

• Length: 1110 bp

>NTDB_id=711409 POF61_RS10140 WP_000264872.1 1991000..1992109(+) (recF) [Streptococcus agalactiae strain CNCTC 10/84 sCas9revertant]
ATGTGGATAAAAAATATTTCTTTAAAACATTATCGTAATTATGAAGAAGCACAAGTAGATTTTTCACCCAACCTCAATAT
TTTCATTGGCCGCAATGCTCAAGGAAAGACAAATTTTTTAGAAGCAATCTACTTCTTGGCTTTGACTCGAAGTCACCGTA
CCCGTTCTGATAAAGAACTTGTACATTTTAAACACCATGATGTACAAATTACAGGTGAGGTAATCCGCAAAAGCGGCCAT
CTTAGCTTAAACATTCAATTATCTGAAAAAGGAAGAATCACTAAGGTTAACCATCTTAAACAGGCAAAATTATCTGATTA
TATTGGGGCTATGACTGTCGTACTTTTTGCCCCTGAAGATCTTCAATTAGTTAAAGGTGCACCTAGTCTAAGACGGAAAT
TTTTAGATATAGATATTGGACAAATTAAGCCAACGTACTTAGCTGAACTTTCAAATTACAATCATGTATTAAAACAAAGA
AACACTTATCTAAAAACTACTAATAACGTCGACAAAACTTTTTTATCAGTCCTTGATGAACAATTAGCTGATTATGGAAG
TCGTGTCATTGAACACCGTTTTGATTTTATCCAAGCCCTAAATGATGAAGCGGACAAACATCACTACATTATCAGTACTG
AGTTAGAACATTTATCTATTCACTACAAATCGTCAATAGAGTTTACAGATAAGTCAAGTATTAGGGAACATTTTTTAAAC
CAATTATCTAAAAGCCACTCAAGAGACATCTTTAAGAAAAACACAAGTATCGGACCTCATCGTGATGATATTACTTTCTT
TATTAATGATATCAATGCTACTTTTGGTAGCCAAGGGCAACAAAGAAGCCTTATTTTATCATTGAAATTAGCAGAAATTG
AGCTAATAAAAACCGTTACCAACGATTATCCGATTCTCTTACTTGACGACGTCATGAGTGAACTTGACAACCATCGTCAA
CTAAAACTCTTAGAGGGAATCAAAGAGAATGTTCAAACATTTATTACTACAACAAGTTTAGAACATTTAAGTGCTTTACC
TGATCAACTCAAAATCTTTAACGTTAGTGATGGTACTATTAGTATTAATGAAAAAAAAGCAACAGATTAA

Domains

Predicted by InterProScan.

• RecF/RecN/SMC N terminal domain (SMC_N) (3-359)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recF	Streptococcus pneumoniae R6	63.636	98.374	0.626
recF	Bacillus subtilis subsp. subtilis str. 168	44.837	99.729	0.447