Detailed information NTG0711302

Bioinformatically predicted

Overview

• Name: recF
• Type: Machinery gene
• Locus tag: POF63_RS10140
• Genome accession: NZ_CP116708
• Coordinates: 1990996..1992105 (+)
• Length: 369 a.a.
• NCBI ID: WP_000264872.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain CNCTC 10/84 sCas9
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1985996..1997105

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF63_RS10115 (POF63_10115)	-	1986368..1987207 (-)	840	WP_000181757.1	energy-coupling factor ABC transporter ATP-binding protein	-
POF63_RS10120 (POF63_10120)	pgsA	1987207..1987749 (-)	543	WP_000239225.1	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	-
POF63_RS10125 (POF63_10125)	-	1987872..1989155 (-)	1284	WP_000217764.1	pitrilysin family protein	-
POF63_RS10130 (POF63_10130)	-	1989157..1990401 (-)	1245	WP_000706188.1	pitrilysin family protein	-
POF63_RS10135 (POF63_10135)	yaaA	1990640..1990993 (+)	354	WP_000384867.1	S4 domain-containing protein YaaA	-
POF63_RS10140 (POF63_10140)	recF	1990996..1992105 (+)	1110	WP_000264872.1	DNA replication/repair protein RecF	Machinery gene
POF63_RS10145 (POF63_10145)	-	1992117..1992983 (-)	867	WP_000398683.1	GRP family sugar transporter	-
POF63_RS10150 (POF63_10150)	-	1993039..1993701 (-)	663	WP_001865840.1	helix-turn-helix transcriptional regulator	-
POF63_RS10155 (POF63_10155)	guaB	1993776..1995257 (-)	1482	WP_000073440.1	IMP dehydrogenase	-
POF63_RS10160 (POF63_10160)	-	1995414..1995899 (-)	486	WP_001034427.1	arginine repressor	-
POF63_RS10165 (POF63_10165)	-	1995909..1996589 (-)	681	WP_000628848.1	Crp/Fnr family transcriptional regulator	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 42470.29 Da
• Isoelectric Point: 7.5270

>NTDB_id=711302 POF63_RS10140 WP_000264872.1 1990996..1992105(+) (recF) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIYFLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGH
LSLNIQLSEKGRITKVNHLKQAKLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKPTYLAELSNYNHVLKQR
NTYLKTTNNVDKTFLSVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHLSIHYKSSIEFTDKSSIREHFLN
QLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFGSQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQ
LKLLEGIKENVQTFITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD

Nucleotide

• Length: 1110 bp

>NTDB_id=711302 POF63_RS10140 WP_000264872.1 1990996..1992105(+) (recF) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGTGGATAAAAAATATTTCTTTAAAACATTATCGTAATTATGAAGAAGCACAAGTAGATTTTTCACCCAACCTCAATAT
TTTCATTGGCCGCAATGCTCAAGGAAAGACAAATTTTTTAGAAGCAATCTACTTCTTGGCTTTGACTCGAAGTCACCGTA
CCCGTTCTGATAAAGAACTTGTACATTTTAAACACCATGATGTACAAATTACAGGTGAGGTAATCCGCAAAAGCGGCCAT
CTTAGCTTAAACATTCAATTATCTGAAAAAGGAAGAATCACTAAGGTTAACCATCTTAAACAGGCAAAATTATCTGATTA
TATTGGGGCTATGACTGTCGTACTTTTTGCCCCTGAAGATCTTCAATTAGTTAAAGGTGCACCTAGTCTAAGACGGAAAT
TTTTAGATATAGATATTGGACAAATTAAGCCAACGTACTTAGCTGAACTTTCAAATTACAATCATGTATTAAAACAAAGA
AACACTTATCTAAAAACTACTAATAACGTCGACAAAACTTTTTTATCAGTCCTTGATGAACAATTAGCTGATTATGGAAG
TCGTGTCATTGAACACCGTTTTGATTTTATCCAAGCCCTAAATGATGAAGCGGACAAACATCACTACATTATCAGTACTG
AGTTAGAACATTTATCTATTCACTACAAATCGTCAATAGAGTTTACAGATAAGTCAAGTATTAGGGAACATTTTTTAAAC
CAATTATCTAAAAGCCACTCAAGAGACATCTTTAAGAAAAACACAAGTATCGGACCTCATCGTGATGATATTACTTTCTT
TATTAATGATATCAATGCTACTTTTGGTAGCCAAGGGCAACAAAGAAGCCTTATTTTATCATTGAAATTAGCAGAAATTG
AGCTAATAAAAACCGTTACCAACGATTATCCGATTCTCTTACTTGACGACGTCATGAGTGAACTTGACAACCATCGTCAA
CTAAAACTCTTAGAGGGAATCAAAGAGAATGTTCAAACATTTATTACTACAACAAGTTTAGAACATTTAAGTGCTTTACC
TGATCAACTCAAAATCTTTAACGTTAGTGATGGTACTATTAGTATTAATGAAAAAAAAGCAACAGATTAA

Domains

Predicted by InterProScan.

• RecF/RecN/SMC N terminal domain (SMC_N) (3-359)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recF	Streptococcus pneumoniae R6	63.636	98.374	0.626
recF	Bacillus subtilis subsp. subtilis str. 168	44.837	99.729	0.447