Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   POF63_RS06415 Genome accession   NZ_CP116708
Coordinates   1237381..1239162 (-) Length   593 a.a.
NCBI ID   WP_001003952.1    Uniprot ID   Q8DZ90
Organism   Streptococcus agalactiae strain CNCTC 10/84 sCas9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1232381..1244162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF63_RS06395 (POF63_06395) - 1232652..1233236 (-) 585 WP_000130596.1 uracil-DNA glycosylase family protein -
  POF63_RS06400 (POF63_06400) pepV 1233333..1234739 (-) 1407 WP_000150956.1 dipeptidase PepV -
  POF63_RS06405 (POF63_06405) - 1234786..1235388 (-) 603 WP_000670189.1 nitroreductase family protein -
  POF63_RS06410 (POF63_06410) - 1235558..1237339 (+) 1782 Protein_1184 glycerophosphodiester phosphodiesterase -
  POF63_RS06415 (POF63_06415) uvrC 1237381..1239162 (-) 1782 WP_001003952.1 excinuclease ABC subunit UvrC Machinery gene
  POF63_RS06420 (POF63_06420) - 1239321..1240088 (+) 768 WP_000567585.1 epoxyqueuosine reductase QueH -
  POF63_RS06425 (POF63_06425) - 1240147..1241487 (-) 1341 WP_000285045.1 MATE family efflux transporter -
  POF63_RS06430 (POF63_06430) - 1241587..1242003 (-) 417 WP_000245056.1 VOC family protein -
  POF63_RS06435 (POF63_06435) - 1242007..1242504 (-) 498 WP_000582587.1 hypothetical protein -
  POF63_RS06440 (POF63_06440) - 1242726..1243322 (+) 597 WP_283833955.1 Ltp family lipoprotein -

Sequence

Protein


Download         Length: 593 a.a.        Molecular weight: 68391.92 Da        Isoelectric Point: 9.5330

>NTDB_id=711268 POF63_RS06415 WP_001003952.1 1237381..1239162(-) (uvrC) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSYFHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEIN
LIQENMPKYNIRLKDDKSYPYIKITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERAAEYRDLIEAISLLRTKQRVIHQ
DMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNMFPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETI
VGCKIVKPQRGEKKQLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGTSPVAAMVV
FVNGKPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLIVIDGGQGQVNIARDVIENQFGLAIPIAGLQ
KNDKHQTHELLFGDPLEVVELPRNSEEFFLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSL
PNIQKAEIEDIIMCGIPRTVAESLRDSLNDPPK

Nucleotide


Download         Length: 1782 bp        

>NTDB_id=711268 POF63_RS06415 WP_001003952.1 1237381..1239162(-) (uvrC) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGAATGAACTGATTAAACATAAGCTAGAATTGTTACCAGACAGTCCTGGTTGTTACCTTCATAAAGATAAGAATGGGAC
AATTATTTATGTAGGTAAGGCTAAAAATCTTAAGAATCGTGTGAAAAGTTATTTTCATGGGAGTCATAATACTAAGACAG
AACTTTTGGTCTCTGAGATTGAAGACTTTGAATACATTGTGACAACATCTAATACCGAAGCTCTATTATTGGAAATTAAT
TTAATCCAAGAAAATATGCCGAAGTATAATATTCGATTGAAAGATGATAAGTCTTACCCATATATCAAAATAACAAATGA
ACGTTATCCTCGTCTTATGATTACACGTCAAGTAAAAAAATCAGATGGCACTTATTTTGGACCTTATCCGGATTCTGGGG
CAGCAACTGAGATTAAGCGGTTACTAGATAGGCTCTTTCCTTTTAAGAAATGTACTAATCCAGCAAATAAAGTATGCTTT
TATTATCATTTAGGGCAATGCAATGCACATACAGTTTGTCAAACTAATAAAGCTTATTGGGATAGTCTGAGAGAAGATGT
AAAACAGTTTCTTAATGGTAAAGATAATAAAATTGTTAACGGATTAACTGAAAAAATGAAGAGCGCTGCTATGACTATGG
AATTTGAGCGTGCGGCTGAATATCGAGATTTGATCGAAGCAATTAGTTTGTTGCGTACTAAGCAGCGTGTTATTCATCAA
GATATGAAGGATAGAGATGTTTTTGGTTATTTCGTTGATAAGGGTTGGATGTGTGTACAGGTCTTTTTTGTTCGAAATGG
AAAATTGATTCAGCGTGACGTGAATATGTTTCCTTATTATAATGAACCAGAGGAAGATTTTCTAACTTACATAGGGCAAT
TTTATCAAGATACAAAACATTTTCTACCCAAAGAAGTCTTTATTCCACAGGATATTGATGCTAAGTCGGTTGAAACAATT
GTAGGCTGTAAGATTGTTAAACCACAACGTGGTGAAAAGAAACAGTTAGTAAATCTGGCAATAAAAAATGCTCGTGTCAG
TTTACAACAGAAGTTTGACTTACTGGAAAAAGATATTCGTAAAACTCATGGGGCAATTGAAAATTTAGGTAATCTTCTTA
ATATCCCTAAACCAGTACGCATAGAAGCATTTGATAATTCCAATATTCAAGGGACAAGTCCAGTAGCTGCTATGGTGGTA
TTTGTGAATGGAAAGCCAAGTAAAAAAGACTATCGAAAGTTTAAAATTAAGACTGTTATCGGACCAGATGATTATGCTAG
TATGAGAGAAGTCATTCATCGGCGTTATAGTAGGGTTTTAAAAGATGGCTTGACACCTCCGGACTTGATTGTAATTGATG
GTGGTCAGGGGCAAGTAAATATAGCGCGTGATGTTATTGAGAACCAATTTGGTTTAGCCATACCTATTGCAGGTTTGCAA
AAGAATGATAAACACCAAACACATGAATTACTTTTTGGAGATCCTTTAGAAGTAGTAGAGCTACCGCGTAATTCAGAAGA
ATTTTTCTTACTTCACCGTATTCAAGATGAGGTACATCGTTTTGCTATTACTTTTCATCGTCAATTACGCTCCAAAAATT
CCTTTTCTTCAAAATTAGATGGTATCACTGGGTTGGGACCAAAACGTAAACAATTGCTAATGAAACATTTTAAAAGCTTA
CCTAATATCCAAAAAGCTGAGATTGAGGACATTATTATGTGTGGTATTCCTAGGACTGTTGCAGAAAGTCTCCGAGATTC
TCTTAATGATCCTCCTAAATAA

Domains

Predicted by InterProScan.

GIY-YIG catalytic domain (GIY-YIG)

(17-90)

UvrB/uvrC motif (UVR)

(197-229)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(372-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DZ90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae TIGR4

76.401

99.325

0.759
  uvrC Streptococcus pneumoniae R6

76.061

99.325

0.755
  uvrC Streptococcus pneumoniae D39

76.061

99.325

0.755