Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   POF62_RS06655 Genome accession   NZ_CP116707
Coordinates   1286226..1288007 (-) Length   593 a.a.
NCBI ID   WP_001003966.1    Uniprot ID   Q3K0N2
Organism   Streptococcus agalactiae strain A909 sCas9revertant     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1281226..1293007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF62_RS06635 (POF62_06635) - 1281496..1282080 (-) 585 WP_000130593.1 uracil-DNA glycosylase family protein -
  POF62_RS06640 (POF62_06640) pepV 1282177..1283583 (-) 1407 WP_000150953.1 dipeptidase PepV -
  POF62_RS06645 (POF62_06645) - 1283630..1284232 (-) 603 WP_000670189.1 nitroreductase family protein -
  POF62_RS06650 (POF62_06650) - 1284402..1286184 (+) 1783 Protein_1231 glycerophosphodiester phosphodiesterase -
  POF62_RS06655 (POF62_06655) uvrC 1286226..1288007 (-) 1782 WP_001003966.1 excinuclease ABC subunit UvrC Machinery gene
  POF62_RS06660 (POF62_06660) - 1288167..1288934 (+) 768 WP_000567582.1 epoxyqueuosine reductase QueH -
  POF62_RS06665 (POF62_06665) - 1288993..1290333 (-) 1341 WP_000285047.1 MATE family efflux transporter -
  POF62_RS06670 (POF62_06670) - 1290434..1290850 (-) 417 WP_000245055.1 VOC family protein -
  POF62_RS06675 (POF62_06675) - 1290854..1291351 (-) 498 WP_000582588.1 hypothetical protein -
  POF62_RS06680 (POF62_06680) - 1291573..1292169 (+) 597 WP_000162275.1 Ltp family lipoprotein -

Sequence

Protein


Download         Length: 593 a.a.        Molecular weight: 68371.88 Da        Isoelectric Point: 9.5360

>NTDB_id=711159 POF62_RS06655 WP_001003966.1 1286226..1288007(-) (uvrC) [Streptococcus agalactiae strain A909 sCas9revertant]
MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVRSYFHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEIN
LIQENMPKYNIRLKDDKSYPYIKITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERAAEYRDLIEAISLLRTKQRVIHQ
DMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNMFPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETI
VGCKIVKPQRGEKKQLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGTSPVAAMVV
FVNGKPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLIVIDGGQGQVNIARDVIENQLGLAIPIAGLQ
KNDKHQTHELLFGDPLEVVELPRNSEEFFLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSL
PNIQKADIEDIIMCGIPRTVAESLRDSLNDPPK

Nucleotide


Download         Length: 1782 bp        

>NTDB_id=711159 POF62_RS06655 WP_001003966.1 1286226..1288007(-) (uvrC) [Streptococcus agalactiae strain A909 sCas9revertant]
ATGAATGAACTGATTAAACATAAGCTAGAATTGTTACCAGACAGTCCTGGTTGTTACCTTCATAAAGATAAGAATGGGAC
AATTATTTATGTAGGTAAGGCTAAAAATCTTAAGAATCGTGTGAGAAGTTATTTTCATGGGAGTCATAATACTAAGACAG
AACTTTTGGTCTCTGAGATTGAAGACTTTGAATACATTGTGACAACATCTAATACCGAAGCTCTATTATTGGAAATTAAT
TTAATCCAAGAAAATATGCCGAAGTATAATATTCGATTAAAAGATGACAAGTCTTACCCATATATCAAAATAACAAATGA
ACGTTATCCTCGTCTTATGATTACACGTCAAGTAAAAAAATCAGATGGCACTTATTTTGGACCTTATCCGGATTCTGGGG
CAGCAACTGAGATTAAGCGGTTACTAGATAGGCTCTTTCCTTTTAAGAAATGTACTAATCCAGCAAATAAAGTATGCTTT
TATTATCATTTAGGGCAATGCAATGCACATACAGTTTGTCAAACTAATAAAGCTTATTGGGATAGTCTGAGAGAAGATGT
AAAACAGTTTCTTAATGGTAAAGATAATAAAATTGTTAACGGATTAACTGAAAAAATGAAGAGCGCTGCTATGACTATGG
AATTTGAGCGTGCGGCTGAATATCGAGATTTGATCGAAGCAATTAGTTTGTTGCGTACTAAGCAGCGTGTTATTCATCAA
GATATGAAGGATAGAGATGTTTTTGGTTATTTCGTTGATAAGGGTTGGATGTGTGTACAGGTCTTTTTTGTTCGAAATGG
AAAATTGATTCAGCGTGACGTGAATATGTTTCCTTATTATAATGAACCAGAGGAAGATTTTCTAACTTACATAGGGCAAT
TTTATCAAGATACAAAACATTTTCTACCCAAAGAAGTCTTTATTCCACAGGATATTGATGCTAAGTCGGTTGAAACAATT
GTAGGCTGTAAGATTGTTAAACCACAACGTGGTGAAAAGAAACAGTTAGTAAATCTGGCAATAAAAAATGCTCGTGTCAG
TTTACAACAGAAGTTTGACTTACTGGAAAAAGATATTCGTAAAACTCATGGGGCAATTGAAAATTTAGGTAATCTTCTTA
ATATCCCTAAACCAGTACGCATAGAAGCATTTGATAATTCCAATATTCAAGGGACAAGTCCAGTAGCTGCTATGGTGGTA
TTTGTGAATGGAAAGCCAAGTAAAAAAGACTATCGAAAGTTTAAAATTAAGACTGTTATCGGACCAGATGATTATGCTAG
TATGAGAGAAGTCATTCATCGGCGTTATAGTAGGGTTTTAAAAGATGGCTTGACACCTCCGGACTTGATTGTAATTGATG
GTGGTCAGGGGCAAGTAAATATAGCGCGTGATGTTATTGAGAACCAATTAGGTTTAGCCATACCTATTGCAGGTTTGCAA
AAGAATGATAAACACCAAACACATGAATTACTTTTTGGAGATCCTTTAGAAGTAGTAGAACTACCGCGTAATTCAGAAGA
ATTTTTCTTACTTCACCGTATTCAAGATGAGGTACATCGTTTTGCTATTACTTTTCATCGTCAATTACGCTCCAAAAATT
CCTTTTCTTCAAAATTAGATGGTATCACTGGGTTGGGACCAAAACGTAAACAATTGCTAATGAAACATTTTAAAAGCTTA
CCTAATATCCAAAAAGCTGATATTGAGGACATTATTATGTGTGGTATTCCTAGGACAGTTGCAGAAAGTCTCCGAGATTC
TCTTAATGATCCTCCTAAATAA

Domains

Predicted by InterProScan.

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(372-523)

UvrB/uvrC motif (UVR)

(197-229)

GIY-YIG catalytic domain (GIY-YIG)

(17-90)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3K0N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae TIGR4

76.74

99.325

0.762
  uvrC Streptococcus pneumoniae R6

76.401

99.325

0.759
  uvrC Streptococcus pneumoniae D39

76.401

99.325

0.759