Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J64_RS03250 Genome accession   NZ_AP018775
Coordinates   638495..639034 (-) Length   179 a.a.
NCBI ID   WP_005654843.1    Uniprot ID   A0A0H3PFD4
Organism   Haemophilus influenzae strain CHBN-III-4 isolate 12H35     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 633495..644034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J64_RS03220 (CHBNIII4_06160) fdx 633898..634239 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J64_RS03225 (CHBNIII4_06170) iscX 634239..634433 (+) 195 WP_014550441.1 Fe-S cluster assembly protein IscX -
  K6J64_RS03230 (CHBNIII4_06180) - 634484..635098 (-) 615 WP_014550442.1 YfgM family protein -
  K6J64_RS03235 (CHBNIII4_06190) hisS 635116..636387 (-) 1272 WP_014550443.1 histidine--tRNA ligase -
  K6J64_RS03240 (CHBNIII4_06200) ispG 636397..637503 (-) 1107 WP_014550444.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J64_RS03245 (CHBNIII4_06210) - 637512..638423 (-) 912 WP_014550445.1 RodZ family helix-turn-helix domain-containing protein -
  K6J64_RS03250 (CHBNIII4_06220) pilF/pilF2 638495..639034 (-) 540 WP_005654843.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J64_RS03255 (CHBNIII4_06230) - 639135..640286 (-) 1152 WP_048946908.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J64_RS03260 (CHBNIII4_06240) - 640533..641411 (+) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K6J64_RS03265 (CHBNIII4_06250) - 641776..642657 (+) 882 WP_048946907.1 metal ABC transporter substrate-binding protein -
  K6J64_RS03270 (CHBNIII4_06260) - 642657..643577 (+) 921 WP_040034370.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20586.20 Da        Isoelectric Point: 8.2626

>NTDB_id=71109 K6J64_RS03250 WP_005654843.1 638495..639034(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-4 isolate 12H35]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=71109 K6J64_RS03250 WP_005654843.1 638495..639034(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-4 isolate 12H35]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCTCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCAAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PFD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment