Detailed information NTG0711086

Bioinformatically predicted

Overview

• Name: recF
• Type: Machinery gene
• Locus tag: POF65_RS10740
• Genome accession: NZ_CP116706
• Coordinates: 2103642..2104751 (+)
• Length: 369 a.a.
• NCBI ID: WP_000264870.1
• Uniprot ID: Q3JYE9
• Organism: Streptococcus agalactiae strain A909 sCas9
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2098642..2109751

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF65_RS10715 (POF65_10715)	-	2099014..2099853 (-)	840	WP_000181757.1	energy-coupling factor ABC transporter ATP-binding protein	-
POF65_RS10720 (POF65_10720)	pgsA	2099853..2100395 (-)	543	WP_000239224.1	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	-
POF65_RS10725 (POF65_10725)	-	2100518..2101801 (-)	1284	WP_000217765.1	pitrilysin family protein	-
POF65_RS10730 (POF65_10730)	-	2101803..2103047 (-)	1245	WP_000706190.1	pitrilysin family protein	-
POF65_RS10735 (POF65_10735)	yaaA	2103286..2103639 (+)	354	WP_000384867.1	S4 domain-containing protein YaaA	-
POF65_RS10740 (POF65_10740)	recF	2103642..2104751 (+)	1110	WP_000264870.1	DNA replication/repair protein RecF	Machinery gene
POF65_RS10745 (POF65_10745)	-	2104763..2105629 (-)	867	WP_000398683.1	GRP family sugar transporter	-
POF65_RS10750 (POF65_10750)	-	2105685..2106347 (-)	663	WP_001871057.1	helix-turn-helix transcriptional regulator	-
POF65_RS10755 (POF65_10755)	guaB	2106422..2107903 (-)	1482	WP_000073440.1	IMP dehydrogenase	-
POF65_RS10760 (POF65_10760)	-	2108060..2108545 (-)	486	WP_001034427.1	arginine repressor	-
POF65_RS10765 (POF65_10765)	-	2108555..2109235 (-)	681	WP_000628848.1	Crp/Fnr family transcriptional regulator	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 42471.27 Da
• Isoelectric Point: 7.3132

>NTDB_id=711086 POF65_RS10740 WP_000264870.1 2103642..2104751(+) (recF) [Streptococcus agalactiae strain A909 sCas9]
MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIYFLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGH
LSLDIQLSEKGRITKVNHLKQAKLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKPTYLAELSNYNHVLKQR
NTYLKTTNNVDKTFLSVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHLSIHYKSSIEFTDKSSIREHFLN
QLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFGSQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQ
LKLLEGIKENVQTFITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD

Nucleotide

• Length: 1110 bp

>NTDB_id=711086 POF65_RS10740 WP_000264870.1 2103642..2104751(+) (recF) [Streptococcus agalactiae strain A909 sCas9]
ATGTGGATAAAAAATATTTCTTTAAAACATTATCGTAATTATGAAGAAGCACAAGTAGATTTTTCACCCAACCTCAATAT
TTTCATTGGCCGCAATGCTCAAGGAAAGACAAATTTTTTAGAAGCAATCTACTTCTTGGCTTTGACTCGAAGTCATCGTA
CCCGTTCTGATAAAGAACTTGTACATTTTAAACACCATGATGTACAAATTACAGGTGAGGTAATCCGCAAAAGCGGCCAT
CTTAGCTTAGATATTCAATTATCTGAAAAAGGAAGAATCACTAAGGTTAACCATCTTAAACAGGCAAAATTATCTGATTA
TATTGGGGCTATGACTGTCGTACTTTTTGCCCCTGAAGATCTTCAATTAGTTAAAGGTGCACCTAGCCTAAGACGGAAAT
TTTTAGATATAGATATTGGACAAATTAAGCCAACGTACTTAGCTGAACTTTCAAATTACAATCATGTATTAAAACAAAGA
AACACTTATCTAAAAACTACTAATAACGTCGACAAAACTTTTTTATCAGTCCTTGATGAACAATTAGCTGATTATGGAAG
TCGTGTCATTGAACACCGTTTTGATTTTATCCAAGCCCTAAATGATGAAGCGGACAAACATCACTACATTATCAGTACTG
AGTTAGAACATTTATCTATTCACTACAAATCGTCAATAGAGTTTACAGATAAGTCAAGTATTAGGGAACATTTTTTAAAC
CAATTATCTAAAAGCCACTCAAGAGACATCTTTAAGAAAAACACAAGCATCGGACCTCATCGTGATGATATTACTTTCTT
TATTAATGATATCAATGCTACTTTTGGTAGCCAAGGGCAACAAAGAAGCCTTATTTTATCATTGAAATTAGCAGAAATTG
AGCTAATAAAAACCGTTACCAACGATTATCCGATTCTCTTACTTGACGACGTCATGAGTGAACTTGACAACCATCGTCAA
CTAAAACTCTTAGAGGGAATCAAAGAGAATGTTCAAACATTTATTACTACAACAAGTTTAGAGCATTTAAGTGCTTTACC
TGATCAACTCAAAATCTTTAATGTTAGTGATGGTACTATTAGTATTAATGAAAAAAAAGCAACAGATTAA

Domains

Predicted by InterProScan.

• RecF/RecN/SMC N terminal domain (SMC_N) (3-359)

3D structure

• AlphaFold DB: Q3JYE9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recF	Streptococcus pneumoniae R6	63.636	98.374	0.626
recF	Bacillus subtilis subsp. subtilis str. 168	44.837	99.729	0.447