Detailed information NTG0710871

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: NNG64_RS12010
• Genome accession: NZ_CP101610
• Coordinates: 2467456..2470956 (-)
• Length: 1166 a.a.
• NCBI ID: WP_060963123.1
• Uniprot ID: A0AAI8N175
• Organism: Bacillus siamensis strain WYJ-E14
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 2462456..2475956

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NNG64_RS12000 (NNG64_11975)	sbcD	2462529..2463701 (-)	1173	WP_047476740.1	exonuclease subunit SbcD	-
NNG64_RS12005 (NNG64_11980)	addA	2463765..2467469 (-)	3705	WP_060965056.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
NNG64_RS12010 (NNG64_11985)	addB	2467456..2470956 (-)	3501	WP_060963123.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
NNG64_RS12015 (NNG64_11990)	-	2471101..2471811 (+)	711	WP_060963124.1	DUF421 domain-containing protein	-
NNG64_RS12020 (NNG64_11995)	-	2471880..2472389 (+)	510	WP_060963125.1	ferritin-like domain-containing protein	-
NNG64_RS12025 (NNG64_12000)	-	2472598..2473221 (+)	624	WP_060963126.1	TetR/AcrR family transcriptional regulator	-
NNG64_RS12030 (NNG64_12005)	-	2473237..2474433 (+)	1197	WP_060963127.1	MFS transporter	-
NNG64_RS12035 (NNG64_12010)	-	2474598..2475755 (+)	1158	WP_016938296.1	AbrB family transcriptional regulator	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 133424.10 Da
• Isoelectric Point: 5.4513

>NTDB_id=710871 NNG64_RS12010 WP_060963123.1 2467456..2470956(-) (addB) [Bacillus siamensis strain WYJ-E14]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKAGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQLPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SGEQAPDELNLFRMTGSTYYKLYQMAKELNADISCKELHGTKRHQHVPELACLESQYDVRPAAAYTGGQEAFTVIQAQNR
RAELEGIAREIQSLVRDGGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPVQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEQMLNETREW
MAAPLVRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQQAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEM
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLRAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGNGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide

• Length: 3501 bp

>NTDB_id=710871 NNG64_RS12010 WP_060963123.1 2467456..2470956(-) (addB) [Bacillus siamensis strain WYJ-E14]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGA
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTGTTGAGGAAGCTGATTGAAGAGCATAAGCACGATTT
TAAAGTGTATCAAAAAGCGAGTGACAAAGCCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGTTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCACTATCTGCATTCAGAGGATTATTT
GACGCTGCTCGCCCAGCAGCTTCCGTTAGCGGATGTTGTAAAAGGCGCCAGAGTTTACGTCGACGGCTTCTATCAATTTA
CACCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGGAGAACAGGCACCGGATGAGCTCAATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGATGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAACGGCATCAGCATGTGCCGGAGCTTGCCTGTTTAG
AATCGCAGTACGATGTGCGTCCCGCTGCGGCGTATACCGGCGGGCAGGAAGCGTTTACCGTCATACAGGCGCAAAACAGA
AGAGCCGAGCTTGAGGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACGGAGGATACCGGTACAGGGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATAGAATTCATCCGTTCAAGTTTGGATGTCGTAAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGTGCAGAAAATAAGAGAGCAGGTCGA
TCAGCTTGAAAACTATTGTATCGCCTACGGAATTAAAGGGGAGCGCTGGACGAACGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAGCAAATGCTGAATGAGACGAGAGAATGG
ATGGCCGCGCCCCTCGTCCGGCTTCAGAACCGGATGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAAGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGGCAGCAGGCCGAAGAGGCCGGAAACATGATCGAAG
CGCAGCAGCACGGCCAGGCATGGGACGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATG
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACCGAATCACTTCATTTTTCTTTAATTCCCCCTGCGCTTGACCA
AGTGTTTGTAGGGAATATGGACCTGTCAAGAATGTACGGCACGTCCTGCACGTTTGTCATCGGGGCCAATGACGGCGTTC
TTCCTGCGCGCCCGGATGAAAACGGCGTGCTGTCCGATGATGACCGGGAGTGGCTGAGAGCGGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GTCATATCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATTGTTGTAAACAGGCTTGGCGAGCTGTTTCCCG
ATCATCAGGAAAAACTGTCGGCTGCTGATCCCGAGCAGGTAAGTGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCACAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTATGAAATCAGTGATGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCACCGGCGCGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGTCCTGTTTCGCGCCAGCTTTACGGCGAGCACATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCTCATTTTGCATCTCACGGGCTGCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTTCACTCCAGCCTCAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAGGAAATTCTGCTCAGTTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTCTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTTGTCCCGATCGGCCTTGAACTCGGTTTCGGCGGAAACGGTCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGCCTGCTGCTTAGAATCGTG
GATTACAAATCAAGCGACAAGGGGCTTGATCTGGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACGTACCTTGA
CTTATCGATTACACATTCAGCGGATTGGCTCGGCATGAAAGCGACCCCCGCAGGCGTTTTGTATTTCCATATCCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTCAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCAATCAGCCTGATGGACACGACTTTGGAGGAAGGACGTTCAAATATCATTAATGCCGGTTT
GAAAAAGGACGGTTCTTTAAGATCCGATTCAGCCGCAGTCAGCGAACGGGATTTTCATCTCCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAATCGGTGTGCCAGTTTGATGAGTCCCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAAAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (305-669)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (791-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	83.276	100	0.833