Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NNG64_RS00585 Genome accession   NZ_CP101610
Coordinates   106832..108211 (+) Length   459 a.a.
NCBI ID   WP_060963752.1    Uniprot ID   A0AAI8HS47
Organism   Bacillus siamensis strain WYJ-E14     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101832..113211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NNG64_RS00565 (NNG64_00565) ctsR 102180..102644 (+) 465 WP_016935919.1 transcriptional regulator CtsR -
  NNG64_RS00570 (NNG64_00570) - 102658..103215 (+) 558 WP_016935918.1 UvrB/UvrC motif-containing protein -
  NNG64_RS00575 (NNG64_00575) - 103215..104306 (+) 1092 WP_060963754.1 protein arginine kinase -
  NNG64_RS00580 (NNG64_00580) clpC 104303..106738 (+) 2436 WP_060963753.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  NNG64_RS00585 (NNG64_00585) radA 106832..108211 (+) 1380 WP_060963752.1 DNA repair protein RadA Machinery gene
  NNG64_RS00590 (NNG64_00590) disA 108215..109297 (+) 1083 WP_003156401.1 DNA integrity scanning diadenylate cyclase DisA -
  NNG64_RS00595 (NNG64_00595) - 109411..110511 (+) 1101 WP_016935914.1 PIN/TRAM domain-containing protein -
  NNG64_RS00600 (NNG64_00600) ispD 110525..111223 (+) 699 WP_060965083.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NNG64_RS00605 (NNG64_00605) ispF 111216..111692 (+) 477 WP_016935912.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49487.82 Da        Isoelectric Point: 6.9565

>NTDB_id=710826 NNG64_RS00585 WP_060963752.1 106832..108211(+) (radA) [Bacillus siamensis strain WYJ-E14]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETVKKEPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQLLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMVIPEANAGGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=710826 NNG64_RS00585 WP_060963752.1 106832..108211(+) (radA) [Bacillus siamensis strain WYJ-E14]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGGAAATGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCGTCAAAAAAGAGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGTGTTCACACCAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGACCCCGGCATCGGGAAATCAACGCT
CCTTCTTCAAGTATCCGCACAATTAGCAGACACGGCCGGCAGCGTGCTTTACATTTCCGGGGAGGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGGCTTGGAATCAACAGCCAATTGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCCGCATTTGTTGTTGTAGACTCCATTCAGACCGTTTACCAAAGCGACATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCTGAATTGATGAAAATCGCGAAAACAAACGGCATTCCGATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCTGGGCCGAGATTGCTTGAGCATATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACAGAAGTGCTGAACCCTTCGGAAATTTTCTTGGAGGAGCGTTCTGCCGGGGCTTCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATTCTGGTTGAAATTCAGGCGCTCATATCACCGACCAGCTTT
GGAAACCCGCGCCGCATGGCTACAGGGATTGACCACAACAGGGTGTCGTTAATCATGGCTGTGCTTGAGAAGCGGGTGGG
GCTATTGCTTCAGAATCAAGATGCGTACTTAAAAGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCTTCAAGCTTCAGAGATACACCGCCGAATCCTGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCGAAACTCGGCTTCAAGCGTATGGTCATACC
TGAGGCGAATGCAGGCGGATGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

92.795

99.782

0.926
  radA Streptococcus pneumoniae Rx1

64.018

98.693

0.632
  radA Streptococcus pneumoniae D39

64.018

98.693

0.632
  radA Streptococcus pneumoniae R6

64.018

98.693

0.632
  radA Streptococcus pneumoniae TIGR4

64.018

98.693

0.632
  radA Streptococcus mitis NCTC 12261

64.018

98.693

0.632
  radA Streptococcus mitis SK321

63.797

98.693

0.63