Detailed information NTG0710644

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NMY32_RS21355
• Genome accession: NZ_CP101560
• Coordinates: 4331466..4332266 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KP67
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4326466..4337266

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMY32_RS21320 (NMY32_21280)	gspH	4326622..4327137 (+)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-
NMY32_RS21325 (NMY32_21285)	gspI	4327134..4327499 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
NMY32_RS21330 (NMY32_21290)	gspJ	4327496..4328092 (+)	597	WP_009309266.1	type II secretion system minor pseudopilin GspJ	-
NMY32_RS21335 (NMY32_21295)	gspK	4328089..4329075 (+)	987	WP_021467522.1	type II secretion system minor pseudopilin GspK	-
NMY32_RS21340 (NMY32_21300)	gspL	4329062..4330255 (+)	1194	WP_048265188.1	type II secretion system protein GspL	-
NMY32_RS21345 (NMY32_21305)	-	4330248..4330733 (+)	486	WP_004145896.1	type II secretion system protein M	-
NMY32_RS21350 (NMY32_21310)	-	4330723..4331469 (+)	747	WP_065888578.1	type II secretion system protein N	-
NMY32_RS21355 (NMY32_21315)	pilD	4331466..4332266 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
NMY32_RS21360 (NMY32_21320)	hrpB	4332314..4334743 (-)	2430	WP_004177388.1	ATP-dependent helicase HrpB	-
NMY32_RS21365 (NMY32_21325)	thpR	4334816..4335352 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
NMY32_RS21370 (NMY32_21330)	sfsA	4335352..4336068 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
NMY32_RS21375 (NMY32_21335)	dksA	4336231..4336686 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=710644 NMY32_RS21355 WP_004145898.1 4331466..4332266(+) (pilD) [Klebsiella pneumoniae subsp. pneumoniae strain KP67]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=710644 NMY32_RS21355 WP_004145898.1 4331466..4332266(+) (pilD) [Klebsiella pneumoniae subsp. pneumoniae strain KP67]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAACTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425