Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J58_RS03275 Genome accession   NZ_AP018772
Coordinates   647767..648306 (-) Length   179 a.a.
NCBI ID   WP_005686810.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-III-1 isolate 15H02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 642767..653306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J58_RS03245 (CHBNIII1_06270) fdx 643170..643511 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J58_RS03250 (CHBNIII1_06280) iscX 643511..643705 (+) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  K6J58_RS03255 (CHBNIII1_06290) - 643756..644370 (-) 615 WP_005686818.1 YfgM family protein -
  K6J58_RS03260 (CHBNIII1_06300) hisS 644388..645659 (-) 1272 WP_005686816.1 histidine--tRNA ligase -
  K6J58_RS03265 (CHBNIII1_06310) ispG 645669..646775 (-) 1107 WP_005686814.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J58_RS03270 (CHBNIII1_06320) - 646784..647695 (-) 912 WP_005686812.1 RodZ family helix-turn-helix domain-containing protein -
  K6J58_RS03275 (CHBNIII1_06330) pilF/pilF2 647767..648306 (-) 540 WP_005686810.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J58_RS03280 (CHBNIII1_06340) - 648407..649558 (-) 1152 WP_005686808.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J58_RS03285 (CHBNIII1_06350) - 649805..650683 (+) 879 WP_005686807.1 serine hydrolase -
  K6J58_RS03290 (CHBNIII1_06360) - 651069..651929 (+) 861 WP_224056855.1 metal ABC transporter substrate-binding protein -
  K6J58_RS03295 (CHBNIII1_06370) - 651929..652849 (+) 921 WP_005686805.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20595.25 Da        Isoelectric Point: 8.6216

>NTDB_id=71003 K6J58_RS03275 WP_005686810.1 647767..648306(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-1 isolate 15H02]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=71003 K6J58_RS03275 WP_005686810.1 647767..648306(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-III-1 isolate 15H02]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTAGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment