Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J66_RS03250 Genome accession   NZ_AP018771
Coordinates   651892..652431 (-) Length   179 a.a.
NCBI ID   WP_038439288.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-8 isolate 10H27     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646892..657431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J66_RS03220 (CHBNII8_06110) fdx 647295..647636 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J66_RS03225 (CHBNII8_06120) iscX 647636..647830 (+) 195 WP_014550441.1 Fe-S cluster assembly protein IscX -
  K6J66_RS03230 (CHBNII8_06130) - 647881..648495 (-) 615 WP_038439292.1 YfgM family protein -
  K6J66_RS03235 (CHBNII8_06140) hisS 648513..649784 (-) 1272 WP_110442563.1 histidine--tRNA ligase -
  K6J66_RS03240 (CHBNII8_06150) ispG 649794..650900 (-) 1107 WP_038439289.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J66_RS03245 (CHBNII8_06160) - 650909..651820 (-) 912 WP_005666610.1 RodZ family helix-turn-helix domain-containing protein -
  K6J66_RS03250 (CHBNII8_06170) pilF/pilF2 651892..652431 (-) 540 WP_038439288.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J66_RS03255 (CHBNII8_06180) - 652532..653683 (-) 1152 WP_005662659.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J66_RS03260 (CHBNII8_06190) - 653930..654808 (+) 879 WP_038439286.1 serine hydrolase -
  K6J66_RS09670 - 654800..654925 (-) 126 WP_257005528.1 hypothetical protein -
  K6J66_RS03265 (CHBNII8_06200) - 655173..656054 (+) 882 WP_038439284.1 metal ABC transporter substrate-binding protein -
  K6J66_RS03270 (CHBNII8_06210) - 656054..656974 (+) 921 WP_005669098.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20583.32 Da        Isoelectric Point: 9.4145

>NTDB_id=70967 K6J66_RS03250 WP_038439288.1 651892..652431(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-8 isolate 10H27]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIKNAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQTDTFENIALCAYSAKKMNIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=70967 K6J66_RS03250 WP_038439288.1 651892..652431(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-8 isolate 10H27]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAAAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAACAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGAATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

94.972

100

0.95
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment