Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NMJ60_RS08485 Genome accession   NZ_CP101314
Coordinates   1726631..1727317 (-) Length   228 a.a.
NCBI ID   WP_001802022.1    Uniprot ID   A0A7U7EYP5
Organism   Staphylococcus aureus strain #492     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1721631..1732317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMJ60_RS08465 rpsT 1722545..1722796 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  NMJ60_RS08470 holA 1722841..1723815 (-) 975 WP_001282563.1 DNA polymerase III subunit delta -
  NMJ60_RS08475 comEC 1723872..1726073 (-) 2202 WP_001557362.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NMJ60_RS08480 - 1726078..1726539 (-) 462 WP_000439693.1 ComE operon protein 2 -
  NMJ60_RS08485 comEA 1726631..1727317 (-) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  NMJ60_RS08490 - 1727357..1728073 (-) 717 WP_044416994.1 class I SAM-dependent DNA methyltransferase -
  NMJ60_RS08495 rsfS 1728076..1728429 (-) 354 WP_001088020.1 ribosome silencing factor -
  NMJ60_RS08500 yqeK 1728430..1729014 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  NMJ60_RS08505 nadD 1729004..1729573 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  NMJ60_RS08510 yhbY 1729576..1729866 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  NMJ60_RS08515 aroE 1729870..1730676 (-) 807 WP_000666757.1 shikimate dehydrogenase -
  NMJ60_RS08520 yqeH 1730690..1731790 (-) 1101 WP_001280137.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25900.65 Da        Isoelectric Point: 9.6751

>NTDB_id=709646 NMJ60_RS08485 WP_001802022.1 1726631..1727317(-) (comEA) [Staphylococcus aureus strain #492]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=709646 NMJ60_RS08485 WP_001802022.1 1726631..1727317(-) (comEA) [Staphylococcus aureus strain #492]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EYP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus N315

100

100

1
  comEA Staphylococcus aureus MW2

97.368

100

0.974