Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   NMT99_RS19320 Genome accession   NZ_CP101290
Coordinates   3728514..3728633 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis strain LjM2     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 3723514..3733633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMT99_RS19300 albG 3723678..3724379 (+) 702 WP_069703620.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  NMT99_RS19305 ywhL 3724385..3725761 (-) 1377 WP_069703619.1 YncE family protein -
  NMT99_RS19310 ywhK 3725800..3727155 (-) 1356 WP_015250986.1 YncE family protein -
  NMT99_RS21215 - 3727152..3727382 (-) 231 WP_069703618.1 hypothetical protein -
  NMT99_RS19315 rapF 3727385..3728530 (+) 1146 WP_029318693.1 response regulator aspartate phosphatase RapF Regulator
  NMT99_RS19320 phrF 3728514..3728633 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  NMT99_RS19325 - 3728703..3728867 (-) 165 WP_248719425.1 hypothetical protein -
  NMT99_RS19330 - 3728788..3729197 (+) 410 Protein_3757 YbaK/EbsC family protein -
  NMT99_RS19335 speB 3729228..3730100 (-) 873 WP_003227545.1 agmatinase -
  NMT99_RS19340 speE 3730161..3730991 (-) 831 WP_003227543.1 spermidine synthase -
  NMT99_RS19345 pbpG 3731194..3733269 (+) 2076 WP_038427996.1 transglycosylase domain-containing protein -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=709511 NMT99_RS19320 WP_009968329.1 3728514..3728633(+) (phrF) [Bacillus subtilis strain LjM2]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=709511 NMT99_RS19320 WP_009968329.1 3728514..3728633(+) (phrF) [Bacillus subtilis strain LjM2]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1

  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487