Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J61_RS03265 Genome accession   NZ_AP018769
Coordinates   656483..657022 (-) Length   179 a.a.
NCBI ID   WP_042611895.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-6 isolate 12H01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 651483..662022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J61_RS03235 (CHBNII6_06130) fdx 651886..652227 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J61_RS03240 (CHBNII6_06140) iscX 652227..652421 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  K6J61_RS03245 (CHBNII6_06150) - 652472..653086 (-) 615 WP_110459378.1 YfgM family protein -
  K6J61_RS03250 (CHBNII6_06160) hisS 653104..654375 (-) 1272 WP_038440737.1 histidine--tRNA ligase -
  K6J61_RS03255 (CHBNII6_06170) ispG 654385..655491 (-) 1107 WP_011272008.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J61_RS03260 (CHBNII6_06180) - 655500..656411 (-) 912 WP_042611894.1 RodZ family helix-turn-helix domain-containing protein -
  K6J61_RS03265 (CHBNII6_06190) pilF/pilF2 656483..657022 (-) 540 WP_042611895.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J61_RS03270 (CHBNII6_06200) - 657123..658274 (-) 1152 WP_042594886.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J61_RS03275 (CHBNII6_06210) - 658521..659399 (+) 879 WP_005659920.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K6J61_RS03280 (CHBNII6_06220) - 659765..660646 (+) 882 WP_110459379.1 metal ABC transporter substrate-binding protein -
  K6J61_RS03285 (CHBNII6_06230) - 660646..661566 (+) 921 WP_005654835.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20598.25 Da        Isoelectric Point: 8.6216

>NTDB_id=70898 K6J61_RS03265 WP_042611895.1 656483..657022(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-6 isolate 12H01]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSTLAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=70898 K6J61_RS03265 WP_042611895.1 656483..657022(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-6 isolate 12H01]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAACACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment