Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NL113_RS09590 Genome accession   NZ_CP101130
Coordinates   1946853..1948232 (-) Length   459 a.a.
NCBI ID   WP_006640292.1    Uniprot ID   M5PBX4
Organism   Bacillus sp. KRF7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1941853..1953232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL113_RS09570 ispF 1943375..1943851 (-) 477 WP_006640296.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  NL113_RS09575 ispD 1943844..1944539 (-) 696 WP_006640295.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NL113_RS09580 - 1944555..1945643 (-) 1089 WP_006640294.1 PIN/TRAM domain-containing protein -
  NL113_RS09585 disA 1945773..1946849 (-) 1077 WP_006640293.1 DNA integrity scanning diadenylate cyclase DisA -
  NL113_RS09590 radA 1946853..1948232 (-) 1380 WP_006640292.1 DNA repair protein RadA Machinery gene
  NL113_RS09595 clpC 1948308..1950740 (-) 2433 WP_006640291.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  NL113_RS09600 - 1950737..1951828 (-) 1092 WP_006640290.1 protein arginine kinase -
  NL113_RS09605 - 1951828..1952385 (-) 558 WP_006640289.1 UvrB/UvrC motif-containing protein -
  NL113_RS09610 - 1952400..1952864 (-) 465 WP_006640288.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49878.26 Da        Isoelectric Point: 7.1320

>NTDB_id=708952 NL113_RS09590 WP_006640292.1 1946853..1948232(-) (radA) [Bacillus sp. KRF7]
MAKTKTKFICQSCGYESPKWMGKCPGCGAWNTMVEETIKKPAANRRTAFSHSVQRVQKPSPISSIETSEEPRIKTNLGEF
NRVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADLNENVLYISGEESVKQTKLRADRLGINSSTLHVLSETDMEYIT
SSIEEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRLAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPHPTDCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMIIPAANLEGWTKPKGIEVVGVENVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=708952 NL113_RS09590 WP_006640292.1 1946853..1948232(-) (radA) [Bacillus sp. KRF7]
ATGGCTAAAACAAAGACTAAATTTATCTGTCAATCATGCGGCTATGAATCTCCAAAATGGATGGGGAAATGCCCGGGCTG
CGGCGCATGGAACACGATGGTCGAAGAAACGATTAAAAAACCGGCCGCCAACAGAAGAACTGCATTTTCTCATTCCGTTC
AAAGGGTTCAAAAACCGTCGCCCATCTCATCAATCGAAACGTCAGAAGAACCGCGGATCAAGACGAATCTCGGCGAATTT
AACCGCGTGCTGGGAAATGGCATCGTCAAAGGCTCGCTTGTCTTAATCGGCGGTGATCCAGGCATCGGAAAATCGACTTT
GCTGCTCCAGGTTTCCGCGCAGCTTGCCGATTTAAATGAAAACGTCCTGTACATATCAGGGGAGGAATCTGTCAAACAAA
CGAAATTAAGAGCGGACCGGTTAGGCATCAACAGTTCAACTCTGCACGTTTTATCTGAAACCGATATGGAGTATATTACG
TCTTCTATAGAAGAGATGAATCCTTCTTTTGTTGTGGTGGATTCAATTCAAACCGTTTATCAAAGCGACATTACCTCAGC
TCCGGGCAGCGTATCCCAAGTGAGGGAATGCACCGCGGAGCTGATGAGACTCGCCAAAACAAAAGGAATTCCGATATTTA
TCGTCGGACATGTTACGAAAGAAGGCTCGATTGCCGGGCCGAGACTGTTGGAGCATATGGTCGACACCGTCCTTTACTTT
GAAGGGGAAAGGCATCACACTTTCCGGATTTTAAGAGCCGTCAAAAACCGATTCGGCTCGACAAATGAAATGGGAATATT
TGAGATGAGGGAAGAGGGCCTTACTGAGGTGCTGAACCCGTCGGAAATTTTTCTTGAAGAACGCTCGGCGGGAGCGGCCG
GGTCAAGTGTTGTAGCATCAATGGAAGGAACGAGGCCGGTTCTGGTCGAAATTCAGGCGCTCATTTCACCTACAAGCTTC
GGAAATCCGCGGAGAATGGCCACCGGCATTGATCATAACCGCGTCTCGCTGCTCATGGCTGTATTGGAAAAAAGAGTCGG
CCTGCTGCTTCAAAATCAGGATGCCTATTTAAAAGTCGCCGGCGGAGTAAAGCTTGATGAACCGGCGATAGATCTTGCCG
TGGCGGTCAGCATTGCTTCAAGCTTTAGAGATACACCTCCCCATCCGACGGATTGTTTTATCGGCGAGGTGGGCCTTACC
GGAGAAGTCCGCAGAGTATCTAGAATCGAACAGCGGGTTCAGGAAGCGGCAAAGCTTGGGTTCAAAAGAATGATTATTCC
CGCCGCAAATTTGGAAGGATGGACCAAACCGAAAGGAATTGAGGTCGTCGGTGTTGAAAATGTAGCTGAGGCTCTTCGAA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M5PBX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

91.703

99.782

0.915
  radA Streptococcus mitis NCTC 12261

63.135

98.693

0.623
  radA Streptococcus pneumoniae Rx1

63.135

98.693

0.623
  radA Streptococcus pneumoniae D39

63.135

98.693

0.623
  radA Streptococcus pneumoniae R6

63.135

98.693

0.623
  radA Streptococcus pneumoniae TIGR4

63.135

98.693

0.623
  radA Streptococcus mitis SK321

62.914

98.693

0.621