Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J56_RS03275 Genome accession   NZ_AP018768
Coordinates   651119..651658 (-) Length   179 a.a.
NCBI ID   WP_011272006.1    Uniprot ID   Q4QNH6
Organism   Haemophilus influenzae strain CHBN-II-5 isolate 13H40     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646119..656658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J56_RS03245 (CHBNII5_06130) fdx 646522..646863 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J56_RS03250 (CHBNII5_06140) iscX 646863..647057 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  K6J56_RS03255 (CHBNII5_06150) - 647108..647722 (-) 615 WP_011272010.1 YfgM family protein -
  K6J56_RS03260 (CHBNII5_06160) hisS 647740..649011 (-) 1272 WP_011272009.1 histidine--tRNA ligase -
  K6J56_RS03265 (CHBNII5_06170) ispG 649021..650127 (-) 1107 WP_011272008.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J56_RS03270 (CHBNII5_06180) - 650136..651047 (-) 912 WP_011272007.1 RodZ family helix-turn-helix domain-containing protein -
  K6J56_RS03275 (CHBNII5_06190) pilF/pilF2 651119..651658 (-) 540 WP_011272006.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J56_RS03280 (CHBNII5_06200) - 651758..652909 (-) 1152 WP_042599461.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J56_RS03285 (CHBNII5_06210) - 653156..654034 (+) 879 WP_038440739.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K6J56_RS03290 (CHBNII5_06220) - 654420..655280 (+) 861 WP_224056855.1 metal ABC transporter substrate-binding protein -
  K6J56_RS03295 (CHBNII5_06230) - 655280..656200 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20554.24 Da        Isoelectric Point: 8.9421

>NTDB_id=70863 K6J56_RS03275 WP_011272006.1 651119..651658(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-5 isolate 13H40]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMDIYQQT
LEKLRQINGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=70863 K6J56_RS03275 WP_011272006.1 651119..651658(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-5 isolate 13H40]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTACATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCAATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4QNH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.089

100

0.961
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment