Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NMD13_RS25625 Genome accession   NZ_CP101034
Coordinates   5527649..5529787 (-) Length   712 a.a.
NCBI ID   WP_370600190.1    Uniprot ID   -
Organism   Pseudomonas nitroreducens strain OT171     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5522649..5534787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD13_RS25610 (NMD13_25505) - 5524267..5525958 (-) 1692 WP_370600188.1 AAA family ATPase -
  NMD13_RS25615 (NMD13_25510) aroB 5525965..5527071 (-) 1107 WP_370600189.1 3-dehydroquinate synthase -
  NMD13_RS25620 (NMD13_25515) aroK 5527122..5527613 (-) 492 WP_370601718.1 shikimate kinase AroK -
  NMD13_RS25625 (NMD13_25520) pilQ 5527649..5529787 (-) 2139 WP_370600190.1 type IV pilus secretin PilQ Machinery gene
  NMD13_RS25630 (NMD13_25525) pilP 5529841..5530365 (-) 525 WP_275608988.1 type 4a pilus biogenesis lipoprotein PilP -
  NMD13_RS25635 (NMD13_25530) pilO 5530362..5531012 (-) 651 WP_275608989.1 type 4a pilus biogenesis protein PilO -
  NMD13_RS25640 (NMD13_25535) pilN 5531009..5531602 (-) 594 WP_275608990.1 type 4a pilus biogenesis protein PilN -
  NMD13_RS25645 (NMD13_25540) pilM 5531602..5532666 (-) 1065 WP_207882126.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 712 a.a.        Molecular weight: 76543.06 Da        Isoelectric Point: 5.1915

>NTDB_id=708291 NMD13_RS25625 WP_370600190.1 5527649..5529787(-) (pilQ) [Pseudomonas nitroreducens strain OT171]
MNKMLSRLSISLLAAVFAPALLAANLQSVDVAALPGDRVELKLGFDGPVATPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIVNLATLVPYTTRSEGSNLYVVVGGAGAASSSYASAAPAPAPVAKPAQPKPYVPAGKAIR
NIDFQRGEQGEGNVVIDLSDPTVSPDIQEQGGKIRLEFPRTQLPDNLRVRLDVKDFATPVQFVNAAAAGDKASITIEPTG
IYDYLVYQTDNRMTVSIKPLTQDDAEKRKKDAFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRKVGNVLLVAPAEEIAARERQELEAQKQISELAPLRRELIQVNYAKAADIAKLFQSVTGVGV
PGAPQAAGADNRGSLTVDDRTNAIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEAAVDYSKSLGVRWGGAFSSGNW
DFYGKDGARTVSDGKLFLPGTDTVGNWTAQDGVAPVPFVDMGVANSTSGIGIGFLSDHAMLDLQLSAMEKSGNGEVVSQP
KVVTSDKETAKILKGSEIPYQEASSSGATSVSFKEAALALEVTPQITPDNRIIMEVKVNKDEPDYANALNGVPPIKKNEV
NAKVLVNDGETIVIGGVFSNTQTKATDKVPFLGDVPFIGRLFRRDTVTDSKNELLVFLTPRIMNNQAFAVGR

Nucleotide


Download         Length: 2139 bp        

>NTDB_id=708291 NMD13_RS25625 WP_370600190.1 5527649..5529787(-) (pilQ) [Pseudomonas nitroreducens strain OT171]
ATGAACAAAATGCTTTCCCGCCTGAGCATCTCCTTGCTCGCGGCAGTCTTTGCGCCGGCTTTGCTGGCGGCCAACCTGCA
GAGCGTCGATGTGGCAGCCCTGCCGGGCGACCGGGTCGAGCTGAAACTGGGCTTCGACGGCCCGGTGGCGACGCCGCGCG
GTTACACCATTGAGCAGCCGGCGCGTATCGCGCTGGATCTGCCGGGTGTGCAGAACAAGCTGGGTAGCAAGAACCGTGAG
CTGGGTGTGGGTAATGCCCGCAGCCTGACGGTGGTTGAGGCGAAAGATCGCACTCGCCTGATCGTCAACCTGGCCACCCT
GGTACCTTATACCACCCGGTCGGAAGGTTCGAATCTGTACGTCGTGGTCGGTGGGGCGGGGGCTGCTTCGAGCTCCTATG
CTTCTGCCGCGCCGGCTCCCGCTCCGGTTGCCAAGCCCGCTCAACCCAAGCCTTACGTTCCGGCCGGCAAGGCGATTCGC
AATATCGACTTCCAGCGTGGCGAACAGGGTGAAGGCAATGTCGTGATCGACCTTTCCGATCCGACCGTCAGCCCGGATAT
CCAGGAGCAGGGCGGCAAGATTCGCCTGGAGTTCCCGCGTACCCAGTTGCCCGACAACCTGCGCGTGCGCCTGGATGTGA
AGGATTTCGCCACGCCGGTGCAGTTCGTCAATGCTGCCGCGGCGGGCGACAAGGCCAGCATCACCATCGAGCCTACCGGC
ATATACGACTATCTCGTCTATCAGACCGACAACCGGATGACCGTCAGCATCAAGCCGCTCACCCAGGATGATGCCGAGAA
GCGCAAGAAGGATGCCTTCGCCTACACCGGTGAGAAGCTGTCGCTGAACTTCCAGGATATCGACGTACGCTCGGTGCTGC
AGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCGTCCGATACCGTTCAGGGCAACATCACCTTGCGTCTGCAGAAT
GTGCCGTGGGATCAGGCGTTGGACCTGGTGCTGAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGTAACGTTCTGCTGGT
TGCGCCGGCGGAAGAGATCGCTGCGCGTGAGCGTCAGGAGCTGGAGGCTCAGAAGCAGATTTCCGAACTGGCTCCTCTGC
GTCGCGAGCTGATCCAGGTGAACTACGCCAAGGCAGCTGATATTGCCAAGCTGTTCCAGTCGGTGACGGGAGTAGGTGTT
CCGGGGGCGCCGCAGGCGGCGGGTGCGGATAATCGTGGTTCGCTCACCGTTGATGATCGCACCAACGCGATCATTGCGTA
CCAGACCCAGGACCGCCTGGATGAGCTGCGCCGTATCGTTGCCCAACTGGATATCCCGGTTCGCCAGGTGATGATCGAAG
CGCGCATCGTCGAGGCCGCTGTGGACTACAGCAAGAGCCTGGGCGTTCGCTGGGGCGGTGCGTTCAGTTCCGGTAATTGG
GACTTCTACGGCAAGGATGGCGCGCGTACGGTCAGTGACGGCAAGCTGTTCCTGCCGGGCACCGACACGGTCGGTAACTG
GACAGCACAGGATGGTGTGGCGCCGGTTCCCTTCGTAGATATGGGGGTGGCCAACAGCACCTCGGGTATCGGTATCGGCT
TCCTGAGCGATCACGCCATGCTTGACCTGCAGCTGTCCGCCATGGAGAAGAGCGGCAACGGTGAAGTGGTCTCCCAGCCG
AAGGTCGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCTCGGAGATCCCCTACCAGGAGGCCAGCTCCAGCGG
TGCTACCTCGGTGTCCTTCAAGGAAGCTGCTCTGGCCCTGGAAGTGACCCCGCAGATCACCCCGGATAACCGCATCATCA
TGGAAGTGAAGGTCAACAAGGATGAGCCGGACTACGCCAACGCGCTGAACGGCGTACCGCCGATCAAGAAGAACGAGGTG
AACGCCAAGGTGCTGGTCAACGATGGCGAGACCATCGTGATTGGTGGTGTGTTCTCCAACACCCAGACCAAGGCTACCGA
CAAGGTTCCGTTCCTGGGCGACGTTCCGTTCATTGGGCGCCTGTTCCGTCGCGATACGGTAACCGACAGCAAGAACGAGC
TGCTGGTTTTCCTTACCCCGCGGATCATGAATAATCAGGCCTTTGCAGTCGGCCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(39-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-449)

Bacterial type II and III secretion system protein (Secretin)

(547-703)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

79.248

100

0.799
  comQ Acinetobacter baylyi ADP1

41.322

100

0.421
  pilQ Acinetobacter baumannii strain A118

40.027

100

0.414
  pilQ Acinetobacter baumannii D1279779

40.027

100

0.414
  pilQ Legionella pneumophila strain ERS1305867

39.156

96.489

0.378
  pilQ Legionella pneumophila strain Lp02

38.649

97.753

0.378