Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K6J55_RS03325 Genome accession   NZ_AP018767
Coordinates   666799..667338 (-) Length   179 a.a.
NCBI ID   WP_042611895.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-II-4 isolate 13H33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 661799..672338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6J55_RS03295 (CHBNII4_06210) fdx 662202..662543 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  K6J55_RS03300 (CHBNII4_06220) iscX 662543..662737 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  K6J55_RS03305 (CHBNII4_06230) - 662788..663402 (-) 615 WP_011272010.1 YfgM family protein -
  K6J55_RS03310 (CHBNII4_06240) hisS 663420..664691 (-) 1272 WP_038440737.1 histidine--tRNA ligase -
  K6J55_RS03315 (CHBNII4_06250) ispG 664701..665807 (-) 1107 WP_011272008.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6J55_RS03320 (CHBNII4_06260) - 665816..666727 (-) 912 WP_042611894.1 RodZ family helix-turn-helix domain-containing protein -
  K6J55_RS03325 (CHBNII4_06270) pilF/pilF2 666799..667338 (-) 540 WP_042611895.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6J55_RS03330 (CHBNII4_06280) - 667439..668590 (-) 1152 WP_042594886.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K6J55_RS03335 (CHBNII4_06290) - 668837..669715 (+) 879 WP_005659920.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K6J55_RS03340 (CHBNII4_06300) - 670102..670962 (+) 861 WP_224056855.1 metal ABC transporter substrate-binding protein -
  K6J55_RS03345 (CHBNII4_06310) - 670962..671882 (+) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20598.25 Da        Isoelectric Point: 8.6216

>NTDB_id=70829 K6J55_RS03325 WP_042611895.1 666799..667338(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-4 isolate 13H33]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSTLAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=70829 K6J55_RS03325 WP_042611895.1 666799..667338(-) (pilF/pilF2) [Haemophilus influenzae strain CHBN-II-4 isolate 13H33]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAACACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment