Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   PF976_RS10040 Genome accession   NZ_CP116011
Coordinates   1931935..1932297 (+) Length   120 a.a.
NCBI ID   WP_013352421.1    Uniprot ID   A0A9P1JH52
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1926935..1937297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS10010 (PF976_10010) - 1927391..1927624 (+) 234 WP_003153997.1 DUF896 domain-containing protein -
  PF976_RS10015 (PF976_10015) recP/tkt 1927791..1929794 (+) 2004 WP_013352417.1 transketolase Machinery gene
  PF976_RS10020 (PF976_10020) sirA 1929945..1930391 (+) 447 WP_013352418.1 sporulation inhibitor of replication protein SirA -
  PF976_RS10025 (PF976_10025) - 1930477..1930695 (+) 219 WP_003153994.1 YneF family protein -
  PF976_RS10030 (PF976_10030) - 1930768..1930941 (-) 174 WP_088030684.1 aspartyl-phosphate phosphatase Spo0E family protein -
  PF976_RS10035 (PF976_10035) - 1931143..1931850 (+) 708 WP_088030531.1 cytochrome c biogenesis protein CcdA -
  PF976_RS10040 (PF976_10040) pilH 1931935..1932297 (+) 363 WP_013352421.1 response regulator Machinery gene
  PF976_RS10045 (PF976_10045) - 1932377..1932868 (+) 492 WP_088030532.1 CcdC family protein -
  PF976_RS10050 (PF976_10050) - 1932896..1933324 (-) 429 WP_147785954.1 DUF2621 domain-containing protein -
  PF976_RS10055 (PF976_10055) - 1933559..1933930 (-) 372 WP_013352424.1 spore coat protein -
  PF976_RS10060 (PF976_10060) - 1934024..1934170 (-) 147 WP_003153984.1 small acid-soluble spore protein P -
  PF976_RS10065 (PF976_10065) sspO 1934200..1934346 (-) 147 WP_003153982.1 acid-soluble spore protein SspO -

Sequence

Protein


Download         Length: 120 a.a.        Molecular weight: 13259.62 Da        Isoelectric Point: 5.2063

>NTDB_id=706756 PF976_RS10040 WP_013352421.1 1931935..1932297(+) (pilH) [Bacillus amyloliquefaciens strain SRCM124317]
MTRVLVVDDAKFMRVKIREILESANYIVAGEAADGEEALAVYQKIRPDLVTMDITMPVKNGIQALQDILAFDPKAHVIMC
TAMRQQRIVLEAIELGAKDFIVKPFEASKVLEAVGRVTGT

Nucleotide


Download         Length: 363 bp        

>NTDB_id=706756 PF976_RS10040 WP_013352421.1 1931935..1932297(+) (pilH) [Bacillus amyloliquefaciens strain SRCM124317]
ATGACACGAGTTTTAGTTGTAGATGACGCCAAGTTCATGAGGGTGAAAATCAGAGAAATTCTGGAATCGGCGAATTACAT
AGTGGCCGGAGAAGCGGCTGACGGCGAGGAAGCCCTGGCGGTATATCAAAAAATCCGTCCCGACCTGGTGACAATGGATA
TTACCATGCCGGTCAAAAACGGTATACAGGCGCTGCAGGATATTTTAGCTTTTGACCCGAAAGCCCATGTCATCATGTGC
ACGGCCATGCGGCAGCAGCGGATTGTTCTGGAAGCGATTGAATTAGGCGCTAAAGACTTTATCGTTAAACCGTTTGAAGC
TTCTAAAGTGCTGGAGGCAGTAGGCAGGGTGACGGGAACGTAA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A9P1JH52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

36.667

100

0.367