Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   PF976_RS06925 Genome accession   NZ_CP116011
Coordinates   1302105..1304201 (-) Length   698 a.a.
NCBI ID   WP_013352040.1    Uniprot ID   A0A9P1JGN0
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1297105..1309201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS06900 (PF976_06900) - 1297680..1297940 (+) 261 WP_013352035.1 aspartyl-phosphate phosphatase Spo0E family protein -
  PF976_RS06905 (PF976_06905) - 1298149..1299666 (-) 1518 WP_088612710.1 ATP-binding protein -
  PF976_RS06910 (PF976_06910) - 1299866..1300312 (+) 447 WP_013352037.1 MarR family winged helix-turn-helix transcriptional regulator -
  PF976_RS06915 (PF976_06915) motB 1300348..1301151 (-) 804 WP_013352038.1 flagellar motor protein MotB -
  PF976_RS06920 (PF976_06920) motA 1301123..1301938 (-) 816 WP_013352039.1 flagellar motor stator protein MotA -
  PF976_RS06925 (PF976_06925) clpE 1302105..1304201 (-) 2097 WP_013352040.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  PF976_RS06930 (PF976_06930) - 1304599..1305636 (+) 1038 WP_013352041.1 membrane protein -
  PF976_RS06940 (PF976_06940) - 1305735..1306857 (+) 1123 Protein_1318 hypothetical protein -
  PF976_RS06945 (PF976_06945) queC 1307109..1307768 (+) 660 WP_003154679.1 7-cyano-7-deazaguanine synthase QueC -
  PF976_RS06950 (PF976_06950) queD 1307829..1308209 (+) 381 WP_232469734.1 6-carboxytetrahydropterin synthase QueD -
  PF976_RS06955 (PF976_06955) queE 1308202..1308933 (+) 732 WP_013352044.1 7-carboxy-7-deazaguanine synthase QueE -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 78004.15 Da        Isoelectric Point: 5.2483

>NTDB_id=706730 PF976_RS06925 WP_013352040.1 1302105..1304201(-) (clpE) [Bacillus amyloliquefaciens strain SRCM124317]
MRCQHCQKNEATIRLNMQVNSIQKQFVLCETCYTELTRKPSMSMGPQSFGFPFEQGFAPDQHKQQAGRKKGLLDELAQNV
TNGAKAGLIDPVIGRDEEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVAN
TGIRGQFEERMKQLMNELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALE
RRFQPVMVHEPSIEQAISILEGIKDKYEKYHSVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLELDTVNE
EEAAERLKAIDAEKTKALQEENYELAAKLRDEEVALEKQLAQESPVSSSEIGAEHIQAIIEQKTGIPVGKLQADEQVKMK
ELEERLHQRVIGQEKAVKKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDSIIRLDMSEYMEK
HAVSKIIGSPPGYVGHDEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVLIMTSNA
GTGEKQTKVGFQSEESVMEEQTLIDSLSSFFKPEFLNRFDSIIEFQSLKKEHLVKIVSLLLAELEDTLKERGISLCVTDD
AKEKIAEIGYHPAFGARPLRRTIQELIEDEMTDLLLDDNEIKAFHVVMEEDDIKVRAQ

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=706730 PF976_RS06925 WP_013352040.1 1302105..1304201(-) (clpE) [Bacillus amyloliquefaciens strain SRCM124317]
ATGCGTTGTCAACATTGTCAGAAAAACGAGGCGACGATTCGCCTGAACATGCAAGTCAATTCCATTCAAAAACAATTCGT
TTTATGTGAAACATGTTATACAGAATTAACCCGAAAACCGTCTATGAGCATGGGACCGCAATCTTTCGGATTCCCGTTTG
AGCAAGGTTTCGCTCCGGATCAACATAAACAGCAGGCCGGCCGTAAAAAAGGCCTGCTCGACGAGCTCGCCCAAAACGTC
ACAAACGGCGCAAAAGCAGGTCTGATTGATCCTGTGATCGGCCGCGATGAAGAAGTGGCGCGCGTCATTGAAATTTTGAA
CCGCCGGAATAAAAACAATCCGGTGCTTATCGGTGAACCGGGTGTCGGTAAAACGGCTATTGCAGAAGGCTTAGCGCTTA
AAATCGCTGAAGGCGATGTGCCGAATAAATTGAAAAACAAAGAGCTTTATCTGCTTGATGTCGCTTCCCTCGTCGCCAAT
ACAGGAATCAGAGGCCAGTTTGAAGAAAGAATGAAACAATTAATGAACGAATTAAAAGAGCGGAAAAACGTCATTTTGTT
CATTGATGAGATTCACCTTCTTGTAGGCGCCGGCTCTGCCGAGGGATCAATGGATGCCGGGAACATCCTGAAACCTGCGC
TTGCCAGAGGCGAACTGCAGGTCATCGGGGCGACGACATTGAAAGAGTACCGCCAGATTGAAAAAGATGCGGCGCTTGAA
AGACGCTTCCAGCCTGTCATGGTTCACGAGCCTTCTATAGAACAAGCCATCAGCATTCTTGAAGGCATTAAAGATAAATA
TGAGAAATACCACAGCGTCACATTCAGCGATGAAGCGATCAAGGCGTGTGTTACGCTTTCTTCCCGCTACATTCAGGACC
GCCACCTGCCGGATAAAGCGATCGACTTGTTAGATGAAGCCGGCTCCAAAGCAAATCTGGAGCTTGATACCGTAAATGAA
GAAGAAGCGGCTGAGCGACTCAAAGCAATTGACGCGGAGAAAACAAAAGCGCTTCAGGAAGAAAATTATGAGCTTGCCGC
GAAACTGCGTGATGAAGAAGTGGCATTGGAAAAGCAGCTGGCACAAGAATCACCGGTCTCTTCTTCAGAAATCGGGGCGG
AGCATATTCAAGCCATCATTGAACAGAAAACAGGCATTCCGGTCGGAAAACTGCAGGCTGATGAACAAGTCAAAATGAAA
GAACTTGAAGAACGCCTTCATCAGCGCGTCATCGGTCAGGAAAAAGCCGTGAAAAAAGTGGCGAAAGCGGTAAGACGAAG
CCGCGCCGGCTTAAAATCCAAAAACAGACCTGTCGGCTCCTTCCTCTTTGTCGGACCGACCGGGGTCGGAAAAACCGAGT
TATCAAAAACACTCGCGGATGAACTTTTCGGCACAAAAGATTCCATCATTCGGCTCGATATGAGTGAATACATGGAAAAA
CACGCCGTTTCAAAAATTATCGGTTCACCGCCGGGATATGTCGGACACGATGAAGCCGGTCAATTAACGGAAAAAGTACG
GCGCAATCCGTACAGCATCGTGCTCCTTGATGAGATTGAAAAAGCGCACCCTGACGTCCAGCATATGTTCCTGCAGATTA
TGGAGGACGGACGTCTGACAGACAGCCAGGGCAGAACGGTCAGCTTTAAAGATACCGTTTTGATCATGACAAGCAATGCG
GGAACCGGCGAAAAACAGACGAAAGTCGGGTTCCAATCTGAAGAAAGCGTTATGGAAGAACAAACGCTGATTGATTCACT
GAGCTCGTTCTTTAAACCTGAATTTTTAAACCGGTTTGACAGCATCATTGAATTCCAATCTCTGAAAAAAGAACATCTCG
TAAAAATCGTCAGTCTGCTTCTCGCAGAACTTGAAGACACATTAAAAGAGCGCGGCATCAGTCTCTGCGTCACAGACGAT
GCGAAAGAAAAAATCGCTGAAATCGGCTACCACCCTGCTTTCGGTGCACGCCCGCTCAGAAGAACGATCCAAGAATTGAT
CGAGGACGAAATGACAGACCTCTTATTGGATGACAATGAAATAAAAGCCTTCCATGTTGTAATGGAAGAGGATGACATCA
AAGTCCGTGCACAATAA

Domains

Predicted by InterProScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(436-602)

AAA lid domain (AAA_lid_9)

(252-351)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(608-679)

ATPase family associated with various cellular activities (AAA) (AAA)

(115-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A9P1JGN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

57.319

100

0.6
  clpC Lactococcus lactis subsp. cremoris KW2

56.369

100

0.596
  clpE Streptococcus pneumoniae TIGR4

55.995

100

0.589
  clpE Streptococcus pneumoniae D39

55.586

100

0.585
  clpE Streptococcus pneumoniae Rx1

55.586

100

0.585
  clpE Streptococcus pneumoniae R6

55.586

100

0.585
  clpC Bacillus subtilis subsp. subtilis str. 168

54.56

91.117

0.497
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.544

90.401

0.43
  clpC Streptococcus pneumoniae TIGR4

46.365

88.682

0.411
  clpC Streptococcus pneumoniae Rx1

46.365

88.682

0.411
  clpC Streptococcus pneumoniae D39

46.365

88.682

0.411
  clpC Streptococcus thermophilus LMD-9

45.455

88.252

0.401
  clpC Streptococcus thermophilus LMG 18311

45.114

87.966

0.397
  clpC Streptococcus mutans UA159

45.114

87.966

0.397
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

41.749

86.819

0.362