Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PG908_RS14735 Genome accession   NZ_CP115957
Coordinates   3106682..3107563 (+) Length   293 a.a.
NCBI ID   WP_029240316.1    Uniprot ID   A0ABX7ZX66
Organism   Ralstonia pseudosolanacearum strain PSS4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3101682..3112563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG908_RS14720 - 3102117..3103394 (+) 1278 WP_011002730.1 HlyC/CorC family transporter -
  PG908_RS14725 pilB 3103642..3105369 (+) 1728 WP_011002731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG908_RS14730 pilC 3105414..3106679 (+) 1266 WP_011002732.1 type II secretion system F family protein Machinery gene
  PG908_RS14735 pilD 3106682..3107563 (+) 882 WP_029240316.1 prepilin peptidase Machinery gene
  PG908_RS14740 coaE 3107576..3108190 (+) 615 WP_011002734.1 dephospho-CoA kinase -
  PG908_RS14745 zapD 3108308..3109066 (+) 759 WP_011002735.1 cell division protein ZapD -
  PG908_RS14750 yacG 3109077..3109292 (+) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  PG908_RS14755 - 3109285..3109746 (-) 462 WP_011002737.1 NUDIX domain-containing protein -
  PG908_RS14760 - 3109724..3110608 (-) 885 WP_011002738.1 ATP-binding protein -
  PG908_RS14765 argJ 3110624..3111853 (-) 1230 WP_011002739.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 293 a.a.        Molecular weight: 31573.54 Da        Isoelectric Point: 8.3415

>NTDB_id=706430 PG908_RS14735 WP_029240316.1 3106682..3107563(+) (pilD) [Ralstonia pseudosolanacearum strain PSS4]
MPGMFVIPTLAQLPAWFVIGAGTLLGLLVGSFLNVVIHRLPRMIEREEANYIAELRNEPLPHPEPYNLVVPRSACPSCGH
QIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFSMFARLPDAVIGATAGYLFLWAIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLVLLSS
VVGLVFGVVRIARGATSESPFSFGPFIAGAGVIALLAGPQLVALTGLAPLLAR

Nucleotide


Download         Length: 882 bp        

>NTDB_id=706430 PG908_RS14735 WP_029240316.1 3106682..3107563(+) (pilD) [Ralstonia pseudosolanacearum strain PSS4]
ATGCCCGGAATGTTCGTCATTCCCACGCTGGCGCAACTGCCGGCGTGGTTCGTGATCGGCGCGGGGACGCTGCTGGGCTT
GCTGGTCGGCAGCTTCCTCAACGTGGTGATTCACCGCCTGCCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCAACGAACCGCTGCCGCATCCCGAGCCGTACAACCTGGTCGTGCCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGCTGAGCTGGCTGGCGTTACGCGGCCGCTGCAGCGCCTGCAAGACCCCCAT
CTCCTGGCGCTATCCCGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGCGCCTGCCTGTGGCACTTCGGACCGACCTGGG
TAGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAACTCCTGCCCGAT
GCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAACCTGTTCAGCATGTTCGCGCGGCTGCCGGACGCGGTAAT
CGGCGCGACGGCGGGGTATCTGTTCCTGTGGGCCATCTACTGGGCCTATAAGCTCCTGCGCGGCCGGGAAGGCATGGGCT
ACGGCGACTTCAAGCTGATGGGCGCGCTGGGCGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTGCTGCTGTCGTCG
GTGGTCGGCCTGGTGTTCGGGGTGGTGCGCATCGCGCGCGGCGCCACGAGCGAATCGCCGTTCTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTGATCGCGCTGCTGGCCGGCCCGCAACTGGTGGCGCTGACGGGGCTCGCGCCACTGCTGGCGCGGT
AA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(24-129)

Type IV leader peptidase family (Peptidase_A24)

(140-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.453

90.444

0.474
  pilD Vibrio campbellii strain DS40M4

48.736

94.539

0.461
  pilD Neisseria gonorrhoeae MS11

48.881

91.468

0.447
  pilD Acinetobacter nosocomialis M2

45.865

90.785

0.416
  pilD Acinetobacter baumannii D1279779

45.865

90.785

0.416