Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PG907_RS14740 Genome accession   NZ_CP115955
Coordinates   3123871..3124752 (+) Length   293 a.a.
NCBI ID   WP_275760905.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2340     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3118871..3129752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG907_RS14725 - 3119306..3120583 (+) 1278 WP_193027557.1 HlyC/CorC family transporter -
  PG907_RS14730 pilB 3120831..3122558 (+) 1728 WP_193027556.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG907_RS14735 pilC 3122603..3123868 (+) 1266 WP_193035781.1 type II secretion system F family protein Machinery gene
  PG907_RS14740 pilD 3123871..3124752 (+) 882 WP_275760905.1 prepilin peptidase Machinery gene
  PG907_RS14745 coaE 3124765..3125379 (+) 615 WP_193027553.1 dephospho-CoA kinase -
  PG907_RS14750 zapD 3125497..3126255 (+) 759 WP_020747465.1 cell division protein ZapD -
  PG907_RS14755 yacG 3126266..3126481 (+) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  PG907_RS14760 - 3126474..3126935 (-) 462 WP_193035777.1 NUDIX domain-containing protein -
  PG907_RS14765 - 3126913..3127797 (-) 885 WP_193035775.1 ATP-binding protein -
  PG907_RS14770 argJ 3127813..3129042 (-) 1230 WP_193035773.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 293 a.a.        Molecular weight: 31654.59 Da        Isoelectric Point: 7.6648

>NTDB_id=706368 PG907_RS14740 WP_275760905.1 3123871..3124752(+) (pilD) [Ralstonia pseudosolanacearum strain RUN2340]
MPGMFVIPTLAELPAWFVIGAGTLLGLLVGSFLNVVIHRLPRMIEREEANYIAELRNEPLPHSEPYNLVVPRSACPSCGH
QIKAVENIPVLSWLALRGRCSACKTPISWRYPAVELVTGVLTGACLWHFGPTWVAVASAALLWFLIAGSMIDADTQLLPD
AITQPLLWLGLLVNLFDMFARLPDAVIGATAGYLFLWSIYWAYKLLRGREGMGYGDFKLMGALGAWFGWQALPLLVLLSS
VVGLVFGVVRIARGATSESPFSFGPFIAGAGVISLLAGPQLVALMGLAPLLAR

Nucleotide


Download         Length: 882 bp        

>NTDB_id=706368 PG907_RS14740 WP_275760905.1 3123871..3124752(+) (pilD) [Ralstonia pseudosolanacearum strain RUN2340]
ATGCCCGGAATGTTCGTCATTCCCACGCTGGCGGAACTGCCGGCGTGGTTCGTGATCGGCGCGGGGACGCTGCTGGGCTT
GTTGGTCGGCAGCTTCCTCAACGTGGTGATTCACCGCCTACCGCGCATGATCGAGCGCGAAGAGGCCAACTACATCGCCG
AGCTGCGCAACGAACCGCTGCCGCATTCCGAGCCGTACAACCTGGTGGTGCCGCGCTCGGCCTGCCCGTCGTGCGGCCAC
CAGATCAAGGCGGTCGAGAACATCCCGGTGCTGAGCTGGCTGGCGTTGCGCGGCCGCTGCAGCGCCTGCAAGACCCCCAT
CTCCTGGCGCTATCCCGCCGTGGAGCTGGTGACCGGCGTGCTGACCGGCGCCTGCCTGTGGCACTTCGGGCCGACCTGGG
TGGCGGTGGCCTCGGCCGCGCTGCTGTGGTTCCTGATCGCCGGCTCGATGATCGACGCCGACACGCAGCTCCTGCCCGAT
GCCATCACCCAGCCGCTGCTGTGGCTCGGCCTGCTGGTGAACCTGTTCGACATGTTCGCCCGGCTGCCGGACGCGGTGAT
CGGCGCGACGGCGGGGTATCTGTTCCTGTGGAGCATCTACTGGGCCTATAAGCTCCTGCGCGGCCGCGAAGGCATGGGCT
ACGGCGACTTCAAGCTGATGGGCGCACTGGGCGCCTGGTTCGGCTGGCAGGCGCTGCCGCTGTTGGTACTGCTGTCGTCG
GTGGTCGGTCTGGTGTTCGGGGTGGTGCGCATCGCGCGCGGCGCCACGAGCGAATCGCCGTTTTCGTTCGGCCCGTTCAT
CGCGGGTGCCGGCGTGATCTCACTGCTGGCCGGCCCGCAATTGGTGGCGCTGATGGGGCTCGCACCACTGCTGGCGCGGT
AA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(140-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(24-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.075

90.444

0.471
  pilD Vibrio campbellii strain DS40M4

49.817

93.174

0.464
  pilD Neisseria gonorrhoeae MS11

48.881

91.468

0.447
  pilD Acinetobacter nosocomialis M2

45.489

90.785

0.413
  pilD Acinetobacter baumannii D1279779

45.489

90.785

0.413