Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   NLL86_RS03495 Genome accession   NZ_CP100660
Coordinates   796988..797854 (-) Length   288 a.a.
NCBI ID   WP_254483063.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain ZL22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 791988..802854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLL86_RS03465 (NLL86_03465) - 792922..794217 (-) 1296 WP_254483060.1 NAD(P)/FAD-dependent oxidoreductase -
  NLL86_RS03470 (NLL86_03470) - 794302..794475 (-) 174 WP_008091951.1 DUF3094 domain-containing protein -
  NLL86_RS03475 (NLL86_03475) - 794608..795237 (-) 630 WP_027918232.1 DUF1780 domain-containing protein -
  NLL86_RS03480 (NLL86_03480) - 795324..796031 (+) 708 WP_254483061.1 energy-coupling factor ABC transporter permease -
  NLL86_RS03485 (NLL86_03485) yacG 796168..796368 (-) 201 WP_054886117.1 DNA gyrase inhibitor YacG -
  NLL86_RS03490 (NLL86_03490) coaE 796365..796988 (-) 624 WP_254483062.1 dephospho-CoA kinase -
  NLL86_RS03495 (NLL86_03495) pilD 796988..797854 (-) 867 WP_254483063.1 A24 family peptidase Machinery gene
  NLL86_RS03500 (NLL86_03500) - 797857..799065 (-) 1209 WP_254483064.1 type II secretion system F family protein -
  NLL86_RS03505 (NLL86_03505) - 799281..799691 (+) 411 WP_254483065.1 pilin -
  NLL86_RS03515 (NLL86_03515) - 799999..801321 (+) 1323 WP_254483067.1 integrase arm-type DNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31651.56 Da        Isoelectric Point: 7.0081

>NTDB_id=706108 NLL86_RS03495 WP_254483063.1 796988..797854(-) (pilD) [Pseudomonas fluorescens strain ZL22]
MTSWTLLAEQPAYFITLAAVLGLLVGSFLNVVVHRLPIMLERQWRREAQEALGLPTTEYERFNLSLPASHCPHCGHAIRA
WENIPVLSYLALRGRCSACKQPISARYPLVELACALLSIVVAWHSGASIEALALLPLTWSLLALSLIDHDQQILPDVIVL
PTLWLGLIVNAFDTVVPLPAALWGAVAGYLSLWSVYWLFKLITGKEGMGYGDFKLLALIGAWGGWQVLPLTLLLSSVIGV
VAGLCLLRMRRQSAGTAIPFGPYLAIAGWIAVLWGDEMYAFYLQLFGM

Nucleotide


Download         Length: 867 bp        

>NTDB_id=706108 NLL86_RS03495 WP_254483063.1 796988..797854(-) (pilD) [Pseudomonas fluorescens strain ZL22]
ATGACTTCATGGACTTTGCTGGCCGAGCAGCCGGCGTACTTCATCACCCTGGCGGCTGTGCTGGGCCTGCTGGTGGGCAG
CTTCCTCAACGTGGTGGTGCATCGCTTGCCGATCATGCTGGAGCGCCAATGGCGGCGTGAGGCGCAGGAAGCATTGGGCC
TGCCGACCACCGAATATGAGCGCTTCAATCTGTCCCTCCCCGCCTCCCACTGCCCGCACTGCGGGCACGCGATCCGCGCC
TGGGAAAACATCCCGGTGCTGAGTTACCTGGCATTGCGCGGGCGCTGCTCGGCCTGCAAGCAGCCGATCAGTGCGCGATA
TCCATTGGTCGAACTGGCCTGTGCGCTGTTGTCGATCGTGGTGGCCTGGCATTCCGGCGCAAGTATCGAGGCGCTGGCCC
TGCTGCCGCTCACCTGGAGCCTGCTGGCACTGAGCCTGATCGATCATGACCAGCAGATTCTGCCCGATGTCATTGTGCTG
CCGACGCTGTGGCTGGGTTTGATCGTCAATGCCTTCGACACCGTGGTGCCGTTGCCCGCTGCACTGTGGGGCGCGGTGGC
CGGGTACCTGAGCCTGTGGTCGGTGTATTGGCTGTTCAAATTGATCACCGGCAAGGAGGGCATGGGCTATGGCGACTTCA
AGCTGCTGGCGCTGATCGGCGCCTGGGGCGGCTGGCAGGTATTGCCGTTGACCTTGCTGCTGTCATCGGTAATCGGCGTG
GTGGCGGGCCTGTGCCTGCTGCGCATGCGCAGGCAATCCGCCGGCACGGCCATACCATTTGGCCCTTATCTGGCGATTGC
GGGGTGGATTGCTGTGCTCTGGGGTGATGAAATGTACGCCTTCTACCTGCAACTGTTTGGAATGTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-124)

Type IV leader peptidase family (Peptidase_A24)

(137-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.235

96.181

0.531
  pilD Vibrio campbellii strain DS40M4

51.812

95.833

0.497
  pilD Acinetobacter baumannii D1279779

51.119

93.056

0.476
  pilD Neisseria gonorrhoeae MS11

50.558

93.403

0.472
  pilD Acinetobacter nosocomialis M2

50.746

93.056

0.472