Detailed information NTG0706064

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NL778_RS21975
• Genome accession: NZ_CP100652
• Coordinates: 4828440..4829306 (+)
• Length: 288 a.a.
• NCBI ID: WP_254466574.1
• Uniprot ID: -
• Organism: Pseudomonas putida strain HSM-C2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4823440..4834306

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NL778_RS25480	-	4823999..4824070 (-)	72	Protein_4327	integrase	-
NL778_RS21955 (NL778_21955)	-	4824964..4826292 (-)	1329	WP_254466570.1	integrase arm-type DNA-binding domain-containing protein	-
NL778_RS21965 (NL778_21965)	-	4826603..4827013 (-)	411	WP_012316337.1	pilin	-
NL778_RS21970 (NL778_21970)	-	4827229..4828437 (+)	1209	WP_254466572.1	type II secretion system F family protein	-
NL778_RS21975 (NL778_21975)	pilD	4828440..4829306 (+)	867	WP_254466574.1	A24 family peptidase	Machinery gene
NL778_RS21980 (NL778_21980)	coaE	4829306..4829929 (+)	624	WP_012316340.1	dephospho-CoA kinase	-
NL778_RS21985 (NL778_21985)	yacG	4829926..4830126 (+)	201	WP_012316341.1	DNA gyrase inhibitor YacG	-
NL778_RS21990 (NL778_21990)	-	4830257..4830997 (-)	741	WP_254466576.1	energy-coupling factor ABC transporter permease	-
NL778_RS21995 (NL778_21995)	-	4831032..4831661 (+)	630	WP_012316343.1	DUF1780 domain-containing protein	-
NL778_RS22000 (NL778_22000)	-	4831794..4831967 (+)	174	WP_012316344.1	DUF3094 domain-containing protein	-
NL778_RS22005 (NL778_22005)	-	4832052..4833347 (+)	1296	WP_012316345.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31707.54 Da
• Isoelectric Point: 6.2130

>NTDB_id=706064 NL778_RS21975 WP_254466574.1 4828440..4829306(+) (pilD) [Pseudomonas putida strain HSM-C2]
MTLWTLLAEQPAYFFTMATVLGLLVGSFINVVAYRLPIMLERQWQREAQEALGLPTDEHERFDLCLPASRCPHCGHAIRA
WENIPVLSYLALRGRCSACKQPIGSRYPLVELACALLSLTVAWHSGAGMEALALLAFTWSLLALSLVDHDKQILPDVLVL
PTLWLGLIVNVFDTVVPLSDAIWGAVAGYLSLWTVYWLFKLITGKEGMGYGDFKLLALIGAWGGWQVVPLTLMLSSLVGA
VVGLCMLRLRSHSMGTAIPFGPYLAIAGWIAVLWGDEIYASYLQLLGF

Nucleotide

• Length: 867 bp

>NTDB_id=706064 NL778_RS21975 WP_254466574.1 4828440..4829306(+) (pilD) [Pseudomonas putida strain HSM-C2]
ATGACTTTATGGACTTTACTGGCCGAACAGCCGGCGTACTTCTTCACTATGGCGACCGTGCTTGGCCTGTTGGTCGGCAG
CTTCATCAATGTGGTGGCGTATCGGCTGCCGATCATGCTGGAGCGCCAATGGCAGCGTGAAGCGCAGGAGGCGCTGGGGC
TGCCGACCGATGAGCACGAGCGCTTCGACCTGTGCCTGCCCGCTTCACGTTGCCCGCACTGTGGGCATGCTATCCGTGCC
TGGGAAAATATTCCGGTGCTCAGTTACCTGGCCTTGCGCGGGCGCTGCTCTGCGTGCAAACAGCCCATCGGCTCACGCTA
CCCGCTGGTGGAGCTGGCCTGCGCCTTGCTGTCGCTGACGGTGGCCTGGCATTCCGGTGCCGGGATGGAGGCACTGGCCT
TGCTGGCCTTTACCTGGAGCCTGCTGGCGCTGAGCCTTGTCGACCACGACAAGCAGATATTGCCGGATGTGCTGGTGCTG
CCCACGCTGTGGCTGGGGCTGATCGTCAATGTTTTCGATACCGTGGTGCCATTGTCCGACGCCATCTGGGGCGCGGTGGC
CGGTTATCTCAGCCTGTGGACAGTGTATTGGCTGTTCAAGCTGATCACCGGCAAGGAAGGCATGGGTTACGGCGACTTCA
AGCTGCTGGCGCTGATCGGGGCCTGGGGCGGCTGGCAGGTAGTGCCGCTGACCTTGATGCTGTCGTCGCTGGTCGGGGCG
GTGGTGGGGTTGTGCATGTTGCGCCTGCGCAGCCATTCGATGGGTACGGCGATACCTTTTGGTCCTTATCTGGCCATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCCTCATACCTGCAACTGCTTGGATTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-124)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.791	96.181	0.517
pilD	Acinetobacter baumannii D1279779	50.178	97.569	0.49
pilD	Acinetobacter nosocomialis M2	49.466	97.569	0.483
pilD	Vibrio campbellii strain DS40M4	50.362	95.833	0.483
pilD	Neisseria gonorrhoeae MS11	47.925	92.014	0.441