Detailed information NTG0705904

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NLI98_RS20830
• Genome accession: NZ_CP100601
• Coordinates: 4287918..4288718 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain WX17388
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4282918..4293718

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NLI98_RS20795 (NLI98_20790)	gspH	4283074..4283589 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
NLI98_RS20800 (NLI98_20795)	gspI	4283586..4283951 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
NLI98_RS20805 (NLI98_20800)	gspJ	4283948..4284544 (+)	597	WP_023342989.1	type II secretion system minor pseudopilin GspJ	-
NLI98_RS20810 (NLI98_20805)	gspK	4284541..4285527 (+)	987	WP_077256163.1	type II secretion system minor pseudopilin GspK	-
NLI98_RS20815 (NLI98_20810)	gspL	4285514..4286707 (+)	1194	WP_088498429.1	type II secretion system protein GspL	-
NLI98_RS20820 (NLI98_20815)	-	4286700..4287185 (+)	486	WP_058229142.1	type II secretion system protein M	-
NLI98_RS20825 (NLI98_20820)	-	4287175..4287921 (+)	747	WP_031588870.1	type II secretion system protein N	-
NLI98_RS20830 (NLI98_20825)	pilD	4287918..4288718 (+)	801	WP_004191960.1	prepilin peptidase	Machinery gene
NLI98_RS20835 (NLI98_20830)	hrpB	4288766..4291195 (-)	2430	WP_004177388.1	ATP-dependent helicase HrpB	-
NLI98_RS20840 (NLI98_20835)	thpR	4291268..4291804 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
NLI98_RS20845 (NLI98_20840)	sfsA	4291804..4292520 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
NLI98_RS20850 (NLI98_20845)	dksA	4292683..4293138 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=705904 NLI98_RS20830 WP_004191960.1 4287918..4288718(+) (pilD) [Klebsiella pneumoniae strain WX17388]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=705904 NLI98_RS20830 WP_004191960.1 4287918..4288718(+) (pilD) [Klebsiella pneumoniae strain WX17388]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGTATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCAGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACTCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421