Detailed information NTG0705620

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NLZ14_RS22630
• Genome accession: NZ_CP100497
• Coordinates: 4819051..4819848 (+)
• Length: 265 a.a.
• NCBI ID: WP_014227970.1
• Uniprot ID: A0A0H3H8P5
• Organism: Klebsiella michiganensis strain LH71
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4814051..4824848

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NLZ14_RS22595 (NLZ14_22595)	gspH	4814192..4814704 (+)	513	WP_046877779.1	type II secretion system minor pseudopilin GspH	-
NLZ14_RS22600 (NLZ14_22600)	gspI	4814701..4815066 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
NLZ14_RS22605 (NLZ14_22605)	gspJ	4815063..4815659 (+)	597	WP_046877781.1	type II secretion system minor pseudopilin GspJ	-
NLZ14_RS22610 (NLZ14_22610)	gspK	4815656..4816636 (+)	981	WP_049083163.1	type II secretion system minor pseudopilin GspK	-
NLZ14_RS22615 (NLZ14_22615)	gspL	4816629..4817822 (+)	1194	WP_254539679.1	type II secretion system protein GspL	-
NLZ14_RS22620 (NLZ14_22620)	gspM	4817815..4818300 (+)	486	WP_025107362.1	type II secretion system protein GspM	-
NLZ14_RS22625 (NLZ14_22625)	-	4818290..4819048 (+)	759	WP_064405742.1	type II secretion system protein N	-
NLZ14_RS22630 (NLZ14_22630)	pilD	4819051..4819848 (+)	798	WP_014227970.1	A24 family peptidase	Machinery gene
NLZ14_RS22635 (NLZ14_22635)	hrpB	4819943..4822372 (-)	2430	WP_049102372.1	ATP-dependent helicase HrpB	-
NLZ14_RS22640 (NLZ14_22640)	thpR	4822445..4822981 (+)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
NLZ14_RS22645 (NLZ14_22645)	sfsA	4822981..4823697 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
NLZ14_RS22650 (NLZ14_22650)	dksA	4823862..4824317 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29166.77 Da
• Isoelectric Point: 8.3358

>NTDB_id=705620 NLZ14_RS22630 WP_014227970.1 4819051..4819848(+) (pilD) [Klebsiella michiganensis strain LH71]
MENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=705620 NLZ14_RS22630 WP_014227970.1 4819051..4819848(+) (pilD) [Klebsiella michiganensis strain LH71]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCGGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0H3H8P5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434