Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   NLK52_RS01930 Genome accession   NZ_CP100436
Coordinates   363377..363826 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain MEC_B298     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 358377..368826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLK52_RS01895 (NLK52_01895) yckA 358522..359202 (-) 681 WP_015382730.1 amino acid ABC transporter permease -
  NLK52_RS01900 (NLK52_01900) yckB 359212..360075 (-) 864 WP_015482776.1 transporter substrate-binding domain-containing protein -
  NLK52_RS01905 (NLK52_01905) - 360237..360434 (+) 198 WP_144052033.1 hypothetical protein -
  NLK52_RS01910 (NLK52_01910) yckC 360484..360915 (+) 432 WP_046340202.1 RDD family protein -
  NLK52_RS01915 (NLK52_01915) yckD 360997..361329 (+) 333 WP_015252849.1 YckD family protein -
  NLK52_RS01920 (NLK52_01920) bglC 361482..362915 (+) 1434 WP_015382735.1 6-phospho-beta-glucosidase -
  NLK52_RS01925 (NLK52_01925) nin/comJ 362952..363350 (-) 399 WP_015382736.1 DNA-entry nuclease inhibitor Regulator
  NLK52_RS01930 (NLK52_01930) nucA/comI 363377..363826 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  NLK52_RS01935 (NLK52_01935) tlpC 363992..365713 (-) 1722 WP_041517645.1 methyl-accepting chemotaxis protein TlpC -
  NLK52_RS01940 (NLK52_01940) hxlB 365824..366381 (-) 558 WP_015482781.1 6-phospho-3-hexuloisomerase -
  NLK52_RS01945 (NLK52_01945) hxlA 366387..367019 (-) 633 WP_015482782.1 3-hexulose-6-phosphate synthase -
  NLK52_RS01950 (NLK52_01950) hxlR 367251..367613 (+) 363 WP_015482783.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=705399 NLK52_RS01930 WP_014475770.1 363377..363826(-) (nucA/comI) [Bacillus subtilis strain MEC_B298]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=705399 NLK52_RS01930 WP_014475770.1 363377..363826(-) (nucA/comI) [Bacillus subtilis strain MEC_B298]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGATATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987