Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NIN90_RS13920 Genome accession   NZ_CP100422
Coordinates   2994523..2995527 (-) Length   334 a.a.
NCBI ID   WP_232658226.1    Uniprot ID   -
Organism   Vibrio furnissii strain 104486766     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2989523..3000527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIN90_RS13895 aroK 2990403..2990921 (-) 519 WP_004728569.1 shikimate kinase AroK -
  NIN90_RS13900 pilQ 2991109..2992833 (-) 1725 WP_004728568.1 type IV pilus secretin PilQ Machinery gene
  NIN90_RS13905 pilP 2992858..2993370 (-) 513 WP_232658223.1 pilus assembly protein PilP Machinery gene
  NIN90_RS13910 pilO 2993360..2993950 (-) 591 WP_232658224.1 type 4a pilus biogenesis protein PilO Machinery gene
  NIN90_RS13915 pilN 2993943..2994533 (-) 591 WP_232658225.1 PilN domain-containing protein Machinery gene
  NIN90_RS13920 pilM 2994523..2995527 (-) 1005 WP_232658226.1 type IV pilus assembly protein PilM Machinery gene
  NIN90_RS13925 - 2995672..2998152 (+) 2481 WP_232658227.1 penicillin-binding protein 1A -
  NIN90_RS13930 oxyR 2998239..2999135 (-) 897 WP_014204332.1 DNA-binding transcriptional regulator OxyR -
  NIN90_RS13935 - 2999279..3000013 (+) 735 WP_004728561.1 glutathione peroxidase -
  NIN90_RS13940 - 3000137..3000400 (+) 264 WP_004728560.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36949.22 Da        Isoelectric Point: 6.5009

>NTDB_id=705087 NIN90_RS13920 WP_232658226.1 2994523..2995527(-) (pilM) [Vibrio furnissii strain 104486766]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSDDIFADNHTLDYQKIVKKLKELKKELPLFSRKVALAVPDNA
VISKVLQIDSDLDASEQEFAVYQAFSHQSPFPIDDLSLDFVPSGDNPAGRSTNTLYQVYATRREVVDNRLSVASKAGFEP
LLLDVQVHGLLQIWQWVSRAQQRDDWMLVDVGLTQTTLCMDFADKAPFYKDIPLGTRQWHADPTPHPVPLSEPPMAGVNE
LVERIARQVQLLLSLQGHQALGGIWLSGGGATTEGLSAALAARLNLPCELFHPLTGFVTRRRMRARVPDDLQRFSTAAGL
ALRGLNWLERDHGA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=705087 NIN90_RS13920 WP_232658226.1 2994523..2995527(-) (pilM) [Vibrio furnissii strain 104486766]
ATGGGTAAATCATTAGTTACAGGTGTCGATATCGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAGGGCGAATC
GTATTCGCTGGTCGGCTACCAAGAAATAGTCGTATCCGACGATATTTTCGCTGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGAATTACCGTTATTTAGTCGCAAAGTTGCGCTGGCTGTGCCGGATAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGCGATTTAGATGCCAGCGAACAAGAATTTGCTGTCTATCAGGCGTTTTCCCA
TCAATCGCCGTTTCCCATCGATGATCTGAGTTTGGATTTCGTACCAAGCGGCGATAACCCCGCCGGGCGCAGCACCAACA
CCCTGTATCAAGTGTACGCCACGCGACGTGAAGTCGTCGATAACCGGTTATCGGTGGCGAGCAAAGCGGGCTTTGAGCCG
CTGCTGCTCGATGTGCAGGTGCATGGGTTGCTCCAGATTTGGCAATGGGTGAGTCGCGCGCAGCAGCGCGACGATTGGAT
GCTGGTGGACGTCGGGCTGACGCAAACCACGTTATGCATGGATTTCGCCGATAAAGCGCCCTTCTATAAAGACATTCCGC
TGGGAACGCGTCAATGGCATGCCGACCCCACGCCCCATCCTGTGCCGCTGAGCGAGCCACCGATGGCTGGAGTCAATGAG
CTAGTTGAGCGAATTGCGCGCCAAGTGCAACTGCTGTTGTCCTTGCAGGGCCATCAAGCGCTGGGCGGGATTTGGCTGTC
GGGCGGTGGGGCGACGACGGAAGGCCTCAGCGCGGCTTTGGCTGCGCGACTGAATCTGCCGTGTGAACTGTTTCATCCAC
TGACGGGATTTGTGACGCGCCGCCGCATGCGAGCGCGTGTACCAGACGATCTGCAACGCTTTAGTACGGCGGCTGGCTTG
GCGCTGCGCGGGCTCAACTGGTTGGAGCGTGACCATGGTGCATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(239-324)

Type IV pilus assembly protein PilM; (PilM_2)

(8-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

64.012

100

0.65
  pilM Vibrio campbellii strain DS40M4

45.562

100

0.461