Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PCS96_RS19505 Genome accession   NZ_CP115330
Coordinates   4057344..4058153 (+) Length   269 a.a.
NCBI ID   WP_000895866.1    Uniprot ID   -
Organism   Escherichia coli strain CUVET16-394     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4052344..4063153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS96_RS19500 (PCS96_19455) sslE 4052649..4057214 (+) 4566 WP_001034531.1 lipoprotein metalloprotease SslE -
  PCS96_RS19505 (PCS96_19460) pilD 4057344..4058153 (+) 810 WP_000895866.1 prepilin peptidase PppA Machinery gene
  PCS96_RS19510 (PCS96_19465) gspS2 4058219..4058629 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  PCS96_RS19515 (PCS96_19470) gspC 4058647..4059606 (+) 960 WP_024190000.1 type II secretion system protein GspC -
  PCS96_RS19520 (PCS96_19475) gspD 4059636..4061696 (+) 2061 WP_000498848.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29419.04 Da        Isoelectric Point: 8.3801

>NTDB_id=703391 PCS96_RS19505 WP_000895866.1 4057344..4058153(+) (pilD) [Escherichia coli strain CUVET16-394]
MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALTFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGALSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALF

Nucleotide


Download         Length: 810 bp        

>NTDB_id=703391 PCS96_RS19505 WP_000895866.1 4057344..4058153(+) (pilD) [Escherichia coli strain CUVET16-394]
ATGCTTTTTGATGTTTTTCAGCAATACCCTGCGGCGATGCCCATACTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGTCCGCATTGCCAGCAGACCATCCGCGTTCGTGACAATATTCCG
CTGCTCTCATGGTTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTTGAGTT
ATTGACAGCACTCACTTTTTTGCTGGCGAGTCTGGTGTGGCCAGAAAGTGGATGGGGGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGACAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACC
GGGCTGATTGCAGCATGGGCGCAACAGAGTCCGTTAACGCTACAAGACGCAGTTACCGGCGTACTGGTGGGATTTATCAC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTCTTCGCTG
CGTTAGGTGGCTGGGTGGGGGCATTATCACTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGTTTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(130-239)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

45.693

99.257

0.454
  pilD Vibrio campbellii strain DS40M4

41.697

100

0.42
  pilD Neisseria gonorrhoeae MS11

39.3

95.539

0.375
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372