Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   NI387_RS01600 Genome accession   NZ_CP099921
Coordinates   337437..338015 (+) Length   192 a.a.
NCBI ID   WP_015297396.1    Uniprot ID   A0A243VIJ8
Organism   Vibrio parahaemolyticus strain BM25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 332437..343015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI387_RS01585 (NI387_01585) oxyR 332760..333668 (+) 909 WP_042771235.1 DNA-binding transcriptional regulator OxyR -
  NI387_RS01590 (NI387_01590) - 333724..336294 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  NI387_RS01595 (NI387_01595) pilM 336437..337453 (+) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  NI387_RS01600 (NI387_01600) pilN 337437..338015 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  NI387_RS01605 (NI387_01605) pilO 338008..338601 (+) 594 WP_005496571.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI387_RS01610 (NI387_01610) pilP 338591..339106 (+) 516 WP_005454781.1 pilus assembly protein PilP Machinery gene
  NI387_RS01615 (NI387_01615) pilQ 339138..340886 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  NI387_RS01620 (NI387_01620) aroK 341075..341593 (+) 519 WP_005381319.1 shikimate kinase AroK -
  NI387_RS01625 (NI387_01625) aroB 341625..342725 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.95 Da        Isoelectric Point: 6.9944

>NTDB_id=702580 NI387_RS01600 WP_015297396.1 337437..338015(+) (pilN) [Vibrio parahaemolyticus strain BM25]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=702580 NI387_RS01600 WP_015297396.1 337437..338015(+) (pilN) [Vibrio parahaemolyticus strain BM25]
GTGCTGTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGCGTCATGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTACCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTCATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCTGACA
GTACACCACGGCTAGCAACCATGCTCGATAACATGGAACGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTTGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTTAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243VIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594