Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NI388_RS01725 Genome accession   NZ_CP099919
Coordinates   364354..364947 (+) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain Isc14B     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359354..369947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI388_RS01710 (NI388_01710) - 360070..362640 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  NI388_RS01715 (NI388_01715) pilM 362783..363799 (+) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  NI388_RS01720 (NI388_01720) pilN 363783..364361 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  NI388_RS01725 (NI388_01725) pilO 364354..364947 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  NI388_RS01730 (NI388_01730) pilP 364937..365452 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  NI388_RS01735 (NI388_01735) pilQ 365484..367232 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  NI388_RS01740 (NI388_01740) aroK 367421..367939 (+) 519 WP_005381319.1 shikimate kinase AroK -
  NI388_RS01745 (NI388_01745) aroB 367971..369071 (+) 1101 WP_308390555.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=702539 NI388_RS01725 WP_005480482.1 364354..364947(+) (pilO) [Vibrio parahaemolyticus strain Isc14B]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=702539 NI388_RS01725 WP_005480482.1 364354..364947(+) (pilO) [Vibrio parahaemolyticus strain Isc14B]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCTAACAAGGTAGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTTACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGCATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726