Detailed information NTG0702465

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PCP71_RS01725
• Genome accession: NZ_CP115294
• Coordinates: 368175..369047 (+)
• Length: 290 a.a.
• NCBI ID: WP_003116251.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 214
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 363175..374047

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP71_RS01700 (PCP71_01700)	-	364074..364316 (+)	243	WP_023082027.1	hypothetical protein	-
PCP71_RS01710 (PCP71_01710)	pilE	364547..365020 (-)	474	WP_015503553.1	pilin	Machinery gene
PCP71_RS01715 (PCP71_01715)	pilB	365251..366951 (+)	1701	WP_003120945.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PCP71_RS01720 (PCP71_01720)	pilC	366955..368172 (+)	1218	WP_010792214.1	type II secretion system F family protein	Machinery gene
PCP71_RS01725 (PCP71_01725)	pilD	368175..369047 (+)	873	WP_003116251.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PCP71_RS01730 (PCP71_01730)	coaE	369044..369655 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
PCP71_RS01735 (PCP71_01735)	yacG	369652..369852 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PCP71_RS01740 (PCP71_01740)	-	369889..370098 (-)	210	WP_003120946.1	hypothetical protein	-
PCP71_RS01745 (PCP71_01745)	-	370204..370893 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
PCP71_RS01750 (PCP71_01750)	-	370890..371360 (-)	471	WP_003125205.1	hypothetical protein	-
PCP71_RS01755 (PCP71_01755)	-	371357..371782 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
PCP71_RS01760 (PCP71_01760)	-	371915..372544 (+)	630	WP_003120948.1	DUF1780 domain-containing protein	-
PCP71_RS01765 (PCP71_01765)	-	372541..372990 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
PCP71_RS01770 (PCP71_01770)	-	373016..373186 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31871.89 Da
• Isoelectric Point: 7.3454

>NTDB_id=702465 PCP71_RS01725 WP_003116251.1 368175..369047(+) (pilD) [Pseudomonas aeruginosa strain 214]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=702465 PCP71_RS01725 WP_003116251.1 368175..369047(+) (pilD) [Pseudomonas aeruginosa strain 214]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCTATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGTGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.017	99.655	0.548
pilD	Vibrio campbellii strain DS40M4	53.497	98.621	0.528
pilD	Acinetobacter nosocomialis M2	50.352	97.931	0.493
pilD	Acinetobacter baumannii D1279779	50.352	97.931	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472