Detailed information NTG0701348

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NH673_RS10520
• Genome accession: NZ_CP099727
• Coordinates: 2340133..2340999 (-)
• Length: 288 a.a.
• NCBI ID: WP_019437919.1
• Uniprot ID: -
• Organism: Pseudomonas putida strain PCL1760
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2335133..2345999

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NH673_RS10485 (NH673_10485)	-	2335523..2336818 (-)	1296	WP_003247549.1	NAD(P)/FAD-dependent oxidoreductase	-
NH673_RS10490 (NH673_10490)	-	2336903..2337076 (-)	174	WP_008091951.1	DUF3094 domain-containing protein	-
NH673_RS10495 (NH673_10495)	-	2337209..2337838 (-)	630	WP_003247546.1	DUF1780 domain-containing protein	-
NH673_RS10500 (NH673_10500)	-	2337894..2338325 (-)	432	WP_014589598.1	hypothetical protein	-
NH673_RS10505 (NH673_10505)	-	2338412..2339119 (+)	708	WP_019437917.1	energy-coupling factor ABC transporter permease	-
NH673_RS10510 (NH673_10510)	yacG	2339313..2339513 (-)	201	WP_014589600.1	DNA gyrase inhibitor YacG	-
NH673_RS10515 (NH673_10515)	coaE	2339510..2340133 (-)	624	WP_019437918.1	dephospho-CoA kinase	-
NH673_RS10520 (NH673_10520)	pilD	2340133..2340999 (-)	867	WP_019437919.1	A24 family peptidase	Machinery gene
NH673_RS10525 (NH673_10525)	-	2341002..2342210 (-)	1209	WP_019437920.1	type II secretion system F family protein	-
NH673_RS10530 (NH673_10530)	-	2342427..2342837 (+)	411	WP_012051131.1	pilin	-
NH673_RS10540 (NH673_10540)	-	2343235..2344390 (+)	1156	Protein_2062	Fic family protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31810.89 Da
• Isoelectric Point: 7.9101

>NTDB_id=701348 NH673_RS10520 WP_019437919.1 2340133..2340999(-) (pilD) [Pseudomonas putida strain PCL1760]
MTLWALLVEQPAYFLTLATVLGLLVGSFINVLVYRLPIMLERQWQREAQEVLGLPTTQHARFDLCLPASRCPHCAHRIRA
WENIPVISYLALGGRCSSCKNRISLRYPVVEVASALLSLVVAWRFGASVEALLALPLTWCLLALSLIDADHQLLPDVLVL
PTMWLGLIVNAFGIHVPLADALWGAVAGYLSLWTVYWVFRLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LVGLCLLRFRRHALGTAIPFGPYLAIAGWIAVLWGDEIYASYIQLLGY

Nucleotide

• Length: 867 bp

>NTDB_id=701348 NH673_RS10520 WP_019437919.1 2340133..2340999(-) (pilD) [Pseudomonas putida strain PCL1760]
ATGACTTTATGGGCTTTGCTGGTTGAACAGCCAGCGTATTTCCTAACCCTTGCCACGGTGCTCGGCCTGCTGGTGGGCAG
CTTTATCAACGTGCTGGTGTACCGCCTGCCGATCATGCTGGAGCGGCAATGGCAGCGTGAGGCGCAAGAGGTATTGGGGC
TGCCGACCACGCAGCACGCGCGTTTCGACCTGTGCCTACCGGCCTCCCGGTGTCCACACTGCGCGCACCGGATTCGTGCG
TGGGAGAACATTCCGGTCATCAGCTACCTGGCCTTGGGCGGGCGCTGTTCGTCCTGCAAAAACCGCATCAGCCTGCGTTA
CCCGGTAGTGGAAGTGGCCAGCGCGCTGCTGTCGCTGGTAGTGGCCTGGCGCTTCGGCGCTTCGGTAGAGGCGTTGCTGG
CTCTGCCGCTGACCTGGTGCCTGCTGGCGCTGAGCCTGATCGATGCGGACCACCAGCTGCTGCCGGATGTGCTGGTGCTG
CCGACCATGTGGCTAGGGCTGATCGTCAATGCCTTTGGCATCCATGTGCCCCTAGCCGACGCACTGTGGGGCGCAGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAGGCTGGTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCA
AGCTGATGGCGCTGATCGGGGCGTGGGGGGGTTGGCAGGTGCTGCCTTTGACGCTGCTGTTGTCGTCGGTGGTCGGGGCG
CTTGTTGGCCTGTGCCTGCTGCGTTTTCGCCGGCATGCCTTGGGCACGGCTATCCCGTTTGGCCCTTATCTGGCAATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCCTCATACATACAACTGCTTGGATACTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.986	96.181	0.5
pilD	Acinetobacter baumannii D1279779	49.11	97.569	0.479
pilD	Acinetobacter nosocomialis M2	48.399	97.569	0.472
pilD	Vibrio campbellii strain DS40M4	49.077	94.097	0.462
pilD	Neisseria gonorrhoeae MS11	48.327	93.403	0.451