Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   NF674_RS22245 Genome accession   NZ_CP099610
Coordinates   5023796..5025385 (+) Length   529 a.a.
NCBI ID   WP_116658819.1    Uniprot ID   A0A2U1R5F2
Organism   Pseudomonas moraviensis strain OTU5VILLAA1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5018796..5030385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF674_RS22225 (NF674_22225) pgeF 5020570..5021319 (-) 750 WP_252875101.1 peptidoglycan editing factor PgeF -
  NF674_RS22230 (NF674_22230) rluD 5021316..5022278 (-) 963 WP_042610487.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  NF674_RS22235 (NF674_22235) - 5022430..5023446 (+) 1017 WP_064364119.1 outer membrane protein assembly factor BamD -
  NF674_RS22240 (NF674_22240) - 5023573..5023806 (+) 234 WP_039761661.1 PP0621 family protein -
  NF674_RS22245 (NF674_22245) pilS 5023796..5025385 (+) 1590 WP_116658819.1 ATP-binding protein Regulator
  NF674_RS22250 (NF674_22250) pilR 5025382..5026728 (+) 1347 WP_252875102.1 sigma-54 dependent transcriptional regulator Regulator
  NF674_RS22255 (NF674_22255) thiO 5026817..5027917 (-) 1101 WP_252875103.1 glycine oxidase ThiO -
  NF674_RS22260 (NF674_22260) - 5027976..5028377 (-) 402 WP_227973093.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58625.22 Da        Isoelectric Point: 6.9043

>NTDB_id=701112 NF674_RS22245 WP_116658819.1 5023796..5025385(+) (pilS) [Pseudomonas moraviensis strain OTU5VILLAA1]
MVAEAANADQKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDDLLRGGSWLYLIINMLLVVFLENTRRPAQLF
TLALVDVLLLCGLFYAAGGVASALGNLLVVSVAISNTLLRRRIGLLIAAIAAIGIVGLSFVLSFSHPLSANAYLQAGTLG
ALCFAASLLVQGLIRRLEVSETLAEQRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSAQSLLGHSHLQGHLID
EYSPALVDRLQLWRNNPTLRPQSLKIPGNGLELQPSFIALEQSPNQQTLVFLEDLAQITQQAQQLKLAALGRLTAGISHE
IRNPLGAISHAAQLLAESEELDSADRRLTQIIQDHSQRMNRVIENVLQLSRRQQSAPQRLDLKPWLEDFVGETREQATER
QHIHLRINSGDFTTLMDPNQLRQILDNLIRNAWRHSAQLHEQAEVWLALFVDPDSQLAVLEVQDNGPGVSADEQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKSRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=701112 NF674_RS22245 WP_116658819.1 5023796..5025385(+) (pilS) [Pseudomonas moraviensis strain OTU5VILLAA1]
GTGGTCGCTGAGGCCGCCAACGCCGACCAGAAACAGGCGCAGCGTCTGCTGCGCCTTTATCACCTGTACCGCTTGAGTGT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAACATGGACAACCGCCTGCTGACGTCGGCCAACGACGATCTGCTGCGCG
GTGGCAGCTGGCTGTATCTGATCATCAATATGCTGCTGGTGGTGTTCCTCGAAAACACCCGGCGCCCGGCGCAACTGTTC
ACTTTAGCGTTGGTCGATGTGCTGCTGTTGTGCGGCCTGTTCTACGCCGCTGGTGGCGTCGCCAGTGCGCTGGGCAATCT
GTTGGTCGTCTCGGTGGCAATCAGCAATACCCTACTGCGCCGGCGCATCGGCCTGCTGATTGCCGCCATCGCGGCGATCG
GCATTGTCGGCCTGAGCTTTGTGCTGAGCTTCAGCCATCCCCTCAGCGCCAACGCCTACCTGCAAGCCGGCACCCTCGGA
GCGCTGTGCTTTGCCGCCTCGTTGCTGGTGCAAGGGCTGATCCGCCGCCTCGAAGTCAGTGAAACCCTCGCCGAACAGCG
CGCCAGTGAAGTGGTTGGCCTGGAAGCGCTCAACGCGTTGATCCTGCAACGCATGCGCACTGGCATTCTGGTGCTCGATG
AGCAACGCCGGGTGCAACTGGCCAATCATAGCGCGCAGAGCCTGCTCGGCCATAGCCACCTGCAAGGTCATTTGATCGAT
GAGTATTCGCCCGCGCTGGTCGATCGCCTGCAACTGTGGCGCAACAACCCGACCTTGCGCCCGCAGAGCCTGAAAATCCC
CGGCAACGGCCTCGAGCTGCAACCAAGCTTCATCGCCCTCGAGCAGAGCCCGAACCAGCAGACCCTGGTGTTTCTCGAAG
ATCTGGCGCAAATCACTCAACAGGCGCAGCAACTCAAACTCGCTGCGCTCGGGCGTTTGACCGCGGGCATCTCCCACGAA
ATTCGCAATCCGTTGGGCGCAATCAGTCATGCCGCGCAGCTGTTGGCCGAGTCCGAGGAACTCGATAGCGCGGATCGGCG
TCTGACGCAGATTATTCAAGATCACTCCCAGCGCATGAACCGAGTCATCGAAAACGTCCTGCAATTGTCCCGCCGTCAGC
AAAGCGCACCGCAACGGCTGGACCTCAAGCCGTGGCTGGAAGACTTCGTCGGCGAAACCCGCGAGCAGGCCACCGAGCGC
CAGCACATTCATCTAAGGATCAATTCGGGAGACTTCACCACGCTGATGGACCCCAACCAGCTGCGGCAGATTCTCGACAA
TCTGATACGTAACGCCTGGCGGCACAGTGCCCAATTGCACGAGCAGGCCGAGGTCTGGCTGGCGCTGTTCGTCGACCCCG
ACAGCCAGTTGGCAGTACTCGAAGTGCAGGACAACGGCCCCGGCGTGTCGGCCGACGAGCAGGCGCATCTGTTCGAACCG
TTCTTCACCACCAGCAGCCAGGGCACCGGTCTTGGCCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCACGCCT
AGACTTCAAATCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-521)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U1R5F2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

59.811

100

0.599