Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NF678_RS22030 Genome accession   NZ_CP099600
Coordinates   4985830..4987047 (+) Length   405 a.a.
NCBI ID   WP_252876913.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6MEDAA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4980830..4992047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF678_RS22010 (NF678_22010) - 4982265..4982564 (+) 300 WP_150742242.1 DUF2845 domain-containing protein -
  NF678_RS22015 (NF678_22015) - 4982829..4983182 (-) 354 WP_071173629.1 BON domain-containing protein -
  NF678_RS22020 (NF678_22020) pilA/pilAI 4983478..4983900 (-) 423 WP_252876912.1 pilin Machinery gene
  NF678_RS22025 (NF678_22025) pilB 4984127..4985827 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF678_RS22030 (NF678_22030) pilC 4985830..4987047 (+) 1218 WP_252876913.1 type II secretion system F family protein Machinery gene
  NF678_RS22035 (NF678_22035) pilD 4987049..4987921 (+) 873 WP_252878912.1 A24 family peptidase Machinery gene
  NF678_RS22040 (NF678_22040) coaE 4987918..4988541 (+) 624 WP_252882697.1 dephospho-CoA kinase -
  NF678_RS22045 (NF678_22045) yacG 4988538..4988738 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  NF678_RS22050 (NF678_22050) - 4988746..4988961 (-) 216 WP_016773148.1 hypothetical protein -
  NF678_RS22055 (NF678_22055) - 4989027..4989716 (-) 690 WP_252882698.1 energy-coupling factor ABC transporter permease -
  NF678_RS22060 (NF678_22060) - 4989907..4990533 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  NF678_RS22065 (NF678_22065) - 4990530..4991057 (+) 528 WP_252882699.1 MOSC domain-containing protein -
  NF678_RS22070 (NF678_22070) - 4991109..4991282 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44085.80 Da        Isoelectric Point: 10.1214

>NTDB_id=700964 NF678_RS22030 WP_252876913.1 4985830..4987047(+) (pilC) [Pseudomonas siliginis strain OTU6MEDAA1]
MAVKAAKVSIYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLTFGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAVFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=700964 NF678_RS22030 WP_252876913.1 4985830..4987047(+) (pilC) [Pseudomonas siliginis strain OTU6MEDAA1]
ATGGCGGTCAAGGCAGCGAAAGTCAGCATTTACGCTTGGGAAGGCACGGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGACAGAACCCCGCACTGATCAAGGCGCAACTGCGCAAACAGGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CGTCCCTGCTGACCTTCGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAACTGGTCGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCTACGGCCCTGCGCAAGAAACCGCAATACTTCGACGAGTTGTACT
GCAATCTGGTCGACGCCGGCGAACAGTCTGGCGCCCTCGACACCTTGCTTGAACGGGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCGGCAGTGGTAACAGGCAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTATTCTCTGGGTTCGGCGCCGAATTGCCGGGCTTTACCCTGATGA
TCATCAGCCTGTCGGAGTTCATGCAGCAGTGGTGGTGGGCGATTCTTTTTGCGCTGGTGGCGGTGTTTTTCGGCACTCGC
CACGCGTTGAAGACTTCGCAGGCCCTGCGCGACCGGCGCGATGCCTGGCTGCTGAAGCTGCCACTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTCGCCCGCTTTGCCCGCACCCTGTCGACCACTTTCGCAGCCGGCGTGCCGCTGGTGGAAGCGCTCG
ATTCGGTCGCCGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCGGTGTTGCGGGTGCGGCAGGACGTCTCGACCGGCATG
CAACTGAATTTCTCCATGCGCAGCACCGGTGTGTTTCCGAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAGTCCGG
GGCACTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAG
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baumannii D1279779

58.824

100

0.593
  pilC Acinetobacter baylyi ADP1

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

53

98.765

0.523
  pilC Vibrio cholerae strain A1552

44.444

97.778

0.435
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.872

100

0.42
  pilC Thermus thermophilus HB27

39.348

98.519

0.388