Detailed information NTG0700381

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NG893_RS20905
• Genome accession: NZ_CP099519
• Coordinates: 4257843..4258643 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain AZJ065
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4252843..4263643

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NG893_RS20870 (NG893_20855)	gspH	4252999..4253508 (+)	510	WP_021441218.1	type II secretion system minor pseudopilin GspH	-
NG893_RS20875 (NG893_20860)	gspI	4253511..4253876 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
NG893_RS20880 (NG893_20865)	gspJ	4253873..4254469 (+)	597	WP_023286045.1	type II secretion system minor pseudopilin GspJ	-
NG893_RS20885 (NG893_20870)	gspK	4254466..4255452 (+)	987	WP_021467522.1	type II secretion system minor pseudopilin GspK	-
NG893_RS20890 (NG893_20875)	gspL	4255439..4256632 (+)	1194	WP_021441216.1	type II secretion system protein GspL	-
NG893_RS20895 (NG893_20880)	-	4256625..4257110 (+)	486	WP_004151943.1	type II secretion system protein M	-
NG893_RS20900 (NG893_20885)	-	4257100..4257846 (+)	747	WP_110111124.1	type II secretion system protein N	-
NG893_RS20905 (NG893_20890)	pilD	4257843..4258643 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
NG893_RS20910 (NG893_20895)	hrpB	4258691..4261120 (-)	2430	WP_023286043.1	ATP-dependent helicase HrpB	-
NG893_RS20915 (NG893_20900)	thpR	4261193..4261729 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
NG893_RS20920 (NG893_20905)	sfsA	4261729..4262445 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
NG893_RS20925 (NG893_20910)	dksA	4262608..4263063 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=700381 NG893_RS20905 WP_004145898.1 4257843..4258643(+) (pilD) [Klebsiella pneumoniae strain AZJ065]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=700381 NG893_RS20905 WP_004145898.1 4257843..4258643(+) (pilD) [Klebsiella pneumoniae strain AZJ065]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425