Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PCP11_RS08550 Genome accession   NZ_CP115265
Coordinates   1810728..1812461 (+) Length   577 a.a.
NCBI ID   WP_024946952.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F022     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1805728..1817461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP11_RS08530 (PCP11_08535) - 1806780..1807532 (+) 753 WP_024947529.1 class I SAM-dependent methyltransferase -
  PCP11_RS08535 (PCP11_08540) - 1807556..1808347 (-) 792 WP_022579960.1 alpha/beta fold hydrolase -
  PCP11_RS08540 (PCP11_08545) - 1808525..1809487 (+) 963 WP_023094659.1 GlxA family transcriptional regulator -
  PCP11_RS08545 (PCP11_08550) - 1809935..1810738 (+) 804 WP_024946951.1 ABC transporter permease -
  PCP11_RS08550 (PCP11_08555) pilF 1810728..1812461 (+) 1734 WP_024946952.1 GspE/PulE family protein Machinery gene
  PCP11_RS08555 (PCP11_08560) - 1812466..1813653 (+) 1188 WP_003138899.1 type II secretion system F family protein -
  PCP11_RS08560 (PCP11_08565) gspG 1813707..1814141 (+) 435 WP_003090519.1 type II secretion system major pseudopilin GspG -
  PCP11_RS08565 (PCP11_08570) - 1814110..1814519 (+) 410 Protein_1694 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PCP11_RS08570 (PCP11_08575) - 1814519..1814944 (+) 426 WP_003110087.1 PulJ/GspJ family protein -
  PCP11_RS08575 (PCP11_08580) - 1814941..1815531 (+) 591 WP_003110086.1 PulJ/GspJ family protein -
  PCP11_RS08580 (PCP11_08585) - 1815521..1816600 (+) 1080 WP_023086106.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63855.95 Da        Isoelectric Point: 5.1633

>NTDB_id=700323 PCP11_RS08550 WP_024946952.1 1810728..1812461(+) (pilF) [Pseudomonas aeruginosa strain F022]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSVRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLCLDRLGMLPDHLRRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVQVHADIGLTFAAALRSILRQDPDVIMIG
EIRDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADL
AATEAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIK
AWLGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=700323 PCP11_RS08550 WP_024946952.1 1810728..1812461(+) (pilF) [Pseudomonas aeruginosa strain F022]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGCCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTGGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGCACCCCGATCAATACCGACTGGTTCGTCGAAGAGCAACT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGGTACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTT
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGTGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCGGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACCACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAGTACCAGGTGCCCAACATCACCCAGGTCCAGGTCCAGG
TCCATGCCGACATCGGCCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGG
GAGATACGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATAC
CAACGATGCGGTGAGTGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTG
TCCAGGCCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTG
GCAGCGACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTG
CGAAGGTACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGC
AGAGAGCTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAG
GCCTGGTTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.725

98.44

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.84

98.614

0.383
  pilF Neisseria gonorrhoeae MS11

36.538

99.133

0.362