Detailed information NTG0699616

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: NE411_RS26170
• Genome accession: NZ_CP099450
• Coordinates: 5032139..5035864 (+)
• Length: 1241 a.a.
• NCBI ID: WP_000970454.1
• Uniprot ID: A0AAP5L5K9
• Organism: Bacillus pacificus strain ANSB901
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 5027139..5040864

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NE411_RS26155 (NE411_26095)	-	5027296..5027889 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
NE411_RS26160 (NE411_26100)	lepB	5027947..5028510 (+)	564	WP_000751900.1	signal peptidase I	-
NE411_RS26165 (NE411_26105)	addB	5028627..5032142 (+)	3516	WP_000058555.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
NE411_RS26170 (NE411_26110)	addA	5032139..5035864 (+)	3726	WP_000970454.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
NE411_RS26175 (NE411_26115)	-	5035877..5036164 (+)	288	WP_000179961.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
NE411_RS26180 (NE411_26120)	gerPF	5036245..5036460 (-)	216	WP_001141566.1	spore germination protein GerPF	-
NE411_RS26185 (NE411_26125)	-	5036503..5036889 (-)	387	WP_000902338.1	spore germination protein GerPE	-
NE411_RS26190 (NE411_26130)	gerPD	5036905..5037099 (-)	195	WP_001102341.1	spore germination protein GerPD	-
NE411_RS26195 (NE411_26135)	gerPC	5037106..5037720 (-)	615	WP_001070749.1	spore germination protein GerPC	-
NE411_RS26200 (NE411_26140)	gerPB	5037791..5037997 (-)	207	WP_001012508.1	spore germination protein GerPB	-
NE411_RS26205 (NE411_26145)	gerPA	5038012..5038233 (-)	222	WP_001111187.1	spore germination protein GerPA	-
NE411_RS26210 (NE411_26150)	-	5038330..5038509 (-)	180	WP_000462840.1	aspartyl-phosphate phosphatase Spo0E family protein	-
NE411_RS26215 (NE411_26155)	-	5038757..5039656 (+)	900	WP_001241718.1	fumarylacetoacetate hydrolase family protein	-
NE411_RS26220 (NE411_26160)	-	5039692..5039976 (-)	285	WP_000926868.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142702.01 Da
• Isoelectric Point: 4.8669

>NTDB_id=699616 NE411_RS26170 WP_000970454.1 5032139..5035864(+) (addA) [Bacillus pacificus strain ANSB901]
MMENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIAGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=699616 NE411_RS26170 WP_000970454.1 5032139..5035864(+) (addA) [Bacillus pacificus strain ANSB901]
ATGATGGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGTTCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCTGGCTCTCAGCACATCCGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCCTTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATAGCGAATCAAACAGAAAATGAACTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATACGATGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCCTACTTATTAGAAGATGTGAAATTCCAACTTGAAACAGCAGAACAGCATATT
CGTAAAGCGACAGAGCTCGCAATGCTCCCTGACGGTCCAGCGCCTCGCGTTGAAACACTGCAAGCGGATTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCATGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGCGATTACAACGAGGATATTGTCAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGTCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTTGTTCAGCTCGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGCAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAGTTTGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATCTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGCTTCACGCAAGAAGGATTAGGCGGCGGAATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGTTATCCAGAAGGTGAAGATGTAGCGGCTGAGCTATTATGTA
TTCAGCAAACGGAAGAAGAGGTAATAGATGGAGAAGAAGGTGCGGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATAATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCACCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCATGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCCTTCTTAAAAGGGGCACCGCTTGAAGAAGAAAAAGAAC
TACATGACAAATTAGAATGGTTCTACAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTAGCTGGACTAGGTCGCCGCTTTAATACACAAGACCTAATGAA
GCGTTTTTTACTGCATAAAGACTTCGGTTTTGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGTGTATTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTGGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGAC
ATCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGGAGCATTCCAGATGAAATTTATGGATACGACACGAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTCGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTATTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGAAGAAGTGTATGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCATCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAATTACGTGCACCAATTCAAACACGTCCTCGTTTTATGGAGAAAAAGGGATTAACATACGCAGAGCGCGG
AACAGCAGTACATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTCGAGCAGGCGGAAGAAATTGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGCGGGGAATCCATTCTTGTCCAAGGGGTTATTGACTGCATGATCGAAGAGGAAGATG
GTATTACGTTAATCGACTTCAAAACGGATACGATTGCAGGGAAATTCCCAGGCGGATTTGATCAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAACTTTCGTTATATGCAAAAGCGCTCGAGAAAAGCTTACAACACCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTGTAAAAATCGAAGAATAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.666	100	0.537