Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   NE411_RS26165 Genome accession   NZ_CP099450
Coordinates   5028627..5032142 (+) Length   1171 a.a.
NCBI ID   WP_000058555.1    Uniprot ID   A0AAP5L6Q6
Organism   Bacillus pacificus strain ANSB901     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5023627..5037142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NE411_RS26140 (NE411_26080) - 5023902..5025122 (+) 1221 WP_225991048.1 sensor histidine kinase -
  NE411_RS26145 (NE411_26085) - 5025177..5025836 (+) 660 WP_042874587.1 response regulator transcription factor -
  NE411_RS26150 (NE411_26090) - 5026111..5026692 (+) 582 WP_000727684.1 hypothetical protein -
  NE411_RS26155 (NE411_26095) - 5027296..5027889 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  NE411_RS26160 (NE411_26100) lepB 5027947..5028510 (+) 564 WP_000751900.1 signal peptidase I -
  NE411_RS26165 (NE411_26105) addB 5028627..5032142 (+) 3516 WP_000058555.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NE411_RS26170 (NE411_26110) addA 5032139..5035864 (+) 3726 WP_000970454.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NE411_RS26175 (NE411_26115) - 5035877..5036164 (+) 288 WP_000179961.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  NE411_RS26180 (NE411_26120) gerPF 5036245..5036460 (-) 216 WP_001141566.1 spore germination protein GerPF -
  NE411_RS26185 (NE411_26125) - 5036503..5036889 (-) 387 WP_000902338.1 spore germination protein GerPE -
  NE411_RS26190 (NE411_26130) gerPD 5036905..5037099 (-) 195 WP_001102341.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134159.23 Da        Isoelectric Point: 5.2121

>NTDB_id=699615 NE411_RS26165 WP_000058555.1 5028627..5032142(+) (addB) [Bacillus pacificus strain ANSB901]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTLDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTIHTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=699615 NE411_RS26165 WP_000058555.1 5028627..5032142(+) (addB) [Bacillus pacificus strain ANSB901]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGCCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAAGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTATTCCAAAAAGCAGCGGAGCAAAATGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAGTTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAGTACTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGCTTCCATTCGT
TTTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTGACAATCACGTTAACGCTAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACGATTCCACTTATGGAACAACCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAGAAATTTAACGGGGAAGCAAGTGTAACGATTCATACAGCCGCGAATTTA
CGAGCGGAAGTAGAAGGCGTTGCTCGTGAAATTCGTAGGCTTGTGGCAGATGAAGACTATCGTTACCGAGATATTGCAGT
TCTTCTTCGTAACGGAGAAAGTTATTACGATGTAATGCGGACTCTATTTACAGATTATAATATCCCGCACTTTATCGATG
AAAAACGCCCGATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTATTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCATACGGTGTACAAGGGAAAAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGAACGGGATGATTACAGACAGCGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCCGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACGGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTTGACGTTCCGAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTTGTTGAGATGCTTGGCGAAGAAAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAATATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTCGTCATTGGAGTGAATGAAGGTG
TGATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAAGAAAGAGATGTTCTTAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTTGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
CATTTCATGCCCGCTTGCAGATGAGGAAGGAAAGACGTTACTTGCTTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAACGACGTAAATGATTTATCACGTTCGGAACAAATTTCATATGTAGCAACACCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACATGGAAGCGATATGGATTTGAAGGCAACTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTTACTTCAGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGCTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATATGGAGACAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTTGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTCAAACTGGATGC
GCCAGATATTGGGGAACTATTCCATGCAGCACTGAAGAGAATTGCGGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGCTGTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAGCA
CGCGAAATCGAGTGGTTTCGTACCAGTTGATTTAGAAGTACCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCAATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGA
ACATTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCGCATACGTGGATGAAAAAAGGCGGCACGGCATCACCAGCTGGTG
TATTGTATTTCCACATTCATAACCCAATTGTGGAGGTAAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCAAGTATTGCAAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGCCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.232

100

0.493