Detailed information NTG0699521

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NE845_RS21775
• Genome accession: NZ_CP099403
• Coordinates: 4360682..4361482 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain 33221
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4355682..4366482

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NE845_RS21740 (NE845_21740)	gspH	4355838..4356353 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
NE845_RS21745 (NE845_21745)	gspI	4356350..4356715 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
NE845_RS21750 (NE845_21750)	gspJ	4356712..4357308 (+)	597	WP_023159595.1	type II secretion system minor pseudopilin GspJ	-
NE845_RS21755 (NE845_21755)	gspK	4357305..4358291 (+)	987	WP_004177385.1	type II secretion system minor pseudopilin GspK	-
NE845_RS21760 (NE845_21760)	gspL	4358278..4359471 (+)	1194	WP_004191957.1	type II secretion system protein GspL	-
NE845_RS21765 (NE845_21765)	-	4359464..4359949 (+)	486	WP_040236726.1	type II secretion system protein M	-
NE845_RS21770 (NE845_21770)	-	4359939..4360685 (+)	747	WP_004178605.1	type II secretion system protein N	-
NE845_RS21775 (NE845_21775)	pilD	4360682..4361482 (+)	801	WP_004191960.1	prepilin peptidase	Machinery gene
NE845_RS21780 (NE845_21780)	hrpB	4361530..4363959 (-)	2430	WP_040236129.1	ATP-dependent helicase HrpB	-
NE845_RS21785 (NE845_21785)	thpR	4364032..4364568 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
NE845_RS21790 (NE845_21790)	sfsA	4364568..4365284 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
NE845_RS21795 (NE845_21795)	dksA	4365447..4365902 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=699521 NE845_RS21775 WP_004191960.1 4360682..4361482(+) (pilD) [Klebsiella pneumoniae strain 33221]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=699521 NE845_RS21775 WP_004191960.1 4360682..4361482(+) (pilD) [Klebsiella pneumoniae strain 33221]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCAGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTATTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGAGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421