Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PCP33_RS16360 Genome accession   NZ_CP115252
Coordinates   3500652..3502379 (+) Length   575 a.a.
NCBI ID   WP_022579957.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F034     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3495652..3507379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP33_RS16340 (PCP33_16345) - 3496363..3497292 (-) 930 WP_126680105.1 LysR family transcriptional regulator -
  PCP33_RS16345 (PCP33_16350) - 3497315..3498289 (-) 975 WP_023120480.1 quinone oxidoreductase family protein -
  PCP33_RS16350 (PCP33_16355) - 3498515..3499267 (+) 753 WP_003110095.1 class I SAM-dependent methyltransferase -
  PCP33_RS16355 (PCP33_16360) - 3499859..3500662 (+) 804 WP_003097732.1 ABC transporter permease -
  PCP33_RS16360 (PCP33_16365) pilF 3500652..3502379 (+) 1728 WP_022579957.1 GspE/PulE family protein Machinery gene
  PCP33_RS16365 (PCP33_16370) - 3502384..3503571 (+) 1188 WP_034084581.1 type II secretion system F family protein -
  PCP33_RS16370 (PCP33_16375) gspG 3503625..3504059 (+) 435 WP_003110089.1 type II secretion system major pseudopilin GspG -
  PCP33_RS16375 (PCP33_16380) - 3504028..3504438 (+) 411 WP_023981094.1 type II secretion system protein -
  PCP33_RS16380 (PCP33_16385) - 3504438..3504863 (+) 426 WP_003110087.1 PulJ/GspJ family protein -
  PCP33_RS16385 (PCP33_16390) - 3504860..3505450 (+) 591 WP_004350096.1 PulJ/GspJ family protein -
  PCP33_RS16390 (PCP33_16395) - 3505440..3506519 (+) 1080 WP_003138906.1 general secretion pathway protein GspK -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63667.71 Da        Isoelectric Point: 5.1633

>NTDB_id=699147 PCP33_RS16360 WP_022579957.1 3500652..3502379(+) (pilF) [Pseudomonas aeruginosa strain F034]
MSSEAVSPGPQVTAEWIPLGQRLLERGLVSGQELERALDLQRRLGGRLGGILVRSGAISENTLMQVLAEQLRLPLVGDDL
RKPSEESIAAFLLSTPINTDWFVEEQLVVWEEGEQLLFAARDPLSPSIRETLGYFYPERSIQAVLCRSQDLDGWLEHSLD
LARQGADRHAYGSEDIRYLRELAEEAPVVELVNNVMSQAMEKRSSDIHIEPREFDFRIRFRVDGVLHDQLSLPRERFAAV
VSRIKLISAIDIAERRLPQDGRMSIRVSGTELDVRVSTLPGVHGESVVMRLLPKEREGLRLDRLGMLPDHLQRMRAWVAE
PHGIILVTGPTGSGKSTTLYGALEEINDGVRKLISVEDPVEYQVPNITQVQVHADIGLTFAAALRSILRQDPDVIMIGEI
RDLETAEIAVQSSLTGHLVLSTLHTNDAVSAFTRLIDMGVEPFLVASPVRGVQAQRLVRRLCAHCAEPCEPTLAAADLAA
TEAATARLFPGQAAQWRVARGCGRCEGTGYSGRVGIYELVDLSSEMRDLIVQRASLEQMHRLAASQGQRNLREDGLIKAW
LGMTSLDEVHRVTSG

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=699147 PCP33_RS16360 WP_022579957.1 3500652..3502379(+) (pilF) [Pseudomonas aeruginosa strain F034]
ATGTCCTCTGAGGCCGTTTCCCCGGGCCCGCAGGTGACGGCCGAGTGGATACCGCTGGGGCAGCGTTTGCTCGAACGCGG
GTTGGTGAGCGGCCAGGAACTCGAGCGGGCGCTCGACCTGCAACGCCGGCTTGGCGGCCGTCTGGGAGGCATTCTCGTGC
GTTCCGGAGCCATCTCGGAAAATACCCTCATGCAGGTGCTGGCGGAGCAACTGCGGTTGCCGCTAGTGGGGGACGACCTG
CGGAAGCCGAGCGAAGAGTCCATCGCCGCATTCCTGCTCAGTACCCCGATCAATACCGACTGGTTCGTCGAAGAACAGTT
GGTGGTCTGGGAGGAGGGCGAGCAACTGCTGTTCGCGGCGCGTGATCCGTTATCTCCTTCGATACGCGAAACCCTGGGCT
ATTTCTATCCGGAGCGCTCGATTCAAGCAGTGCTGTGCCGCTCCCAGGATCTGGACGGCTGGTTGGAGCATTCCCTGGAC
CTGGCCCGGCAAGGTGCGGATCGGCACGCCTACGGCAGCGAAGACATCCGCTACCTGCGGGAGCTGGCGGAGGAGGCGCC
GGTAGTCGAACTCGTCAACAACGTGATGAGCCAGGCCATGGAAAAGCGCTCTTCGGATATTCACATCGAACCCCGGGAGT
TCGACTTCCGTATCCGCTTCCGCGTCGACGGCGTCCTGCACGACCAACTGAGCCTTCCCCGCGAGCGATTCGCGGCGGTT
GTCTCGCGGATCAAGCTGATCTCCGCCATCGACATCGCGGAGCGACGGTTGCCGCAGGATGGACGCATGAGCATCCGGGT
CAGCGGTACGGAGCTCGACGTGCGGGTCTCCACCTTGCCAGGGGTGCATGGCGAATCGGTGGTCATGCGCCTGTTGCCGA
AGGAGCGCGAGGGGTTGCGCCTGGACCGGCTCGGGATGCTCCCGGACCACTTGCAGAGGATGCGTGCATGGGTAGCGGAA
CCCCACGGCATCATCCTGGTGACCGGCCCCACCGGATCGGGCAAGTCCACGACGCTCTATGGCGCGCTGGAGGAAATCAA
TGATGGCGTGCGCAAGCTCATCAGCGTGGAAGATCCCGTGGAGTACCAGGTGCCCAACATCACCCAGGTCCAGGTCCATG
CCGACATCGGTCTGACCTTCGCTGCCGCCTTGCGCTCGATCCTGCGGCAGGATCCCGATGTGATCATGATCGGGGAGATA
CGCGACCTGGAGACGGCGGAGATCGCCGTACAGTCCTCGCTCACCGGTCACCTGGTGCTTTCGACCCTGCATACCAACGA
TGCGGTGAGTGCCTTCACGCGCTTGATCGACATGGGCGTGGAACCCTTCCTGGTGGCTTCGCCGGTGCGCGGTGTCCAGG
CCCAGCGACTGGTGCGCAGGTTGTGCGCCCACTGCGCGGAACCTTGCGAGCCAACCCTTGCTGCGGCCGATCTGGCAGCG
ACCGAGGCGGCCACCGCGCGGCTGTTCCCGGGGCAGGCAGCGCAATGGCGCGTGGCGCGAGGCTGCGGCCGCTGCGAAGG
TACCGGCTACAGCGGCCGTGTGGGCATCTACGAACTCGTCGATCTGTCTTCCGAGATGCGCGACCTGATCGTGCAGAGAG
CTTCCCTCGAACAGATGCATCGGCTTGCCGCCAGCCAGGGACAGCGCAATCTGCGTGAAGATGGCTTGATCAAGGCCTGG
TTGGGTATGACCAGTCTTGACGAGGTGCATCGCGTGACCAGCGGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(194-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

41.696

98.435

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.801

98.609

0.383
  pilF Neisseria gonorrhoeae MS11

36.842

99.13

0.365